Sökning: onr:"swepub:oai:lup.lub.lu.se:faf81412-47bc-4956-83c0-8645bd716430" >
Conditional ablatio...
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Clausen, Bettina HjelmUniversity of Southern Denmark
(författare)
Conditional ablation of myeloid TNF increases lesion volume after experimental stroke in mice, possibly via altered ERK1/2 signaling
- Artikel/kapitelEngelska2016
Förlag, utgivningsår, omfång ...
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2016-07-07
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Springer Science and Business Media LLC,2016
Nummerbeteckningar
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LIBRIS-ID:oai:lup.lub.lu.se:faf81412-47bc-4956-83c0-8645bd716430
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https://lup.lub.lu.se/record/faf81412-47bc-4956-83c0-8645bd716430URI
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https://doi.org/10.1038/srep29291DOI
Kompletterande språkuppgifter
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Språk:engelska
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Sammanfattning på:engelska
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Klassifikation
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Ämneskategori:art swepub-publicationtype
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Ämneskategori:ref swepub-contenttype
Anmärkningar
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Microglia are activated following cerebral ischemia and increase their production of the neuro- and immunomodulatory cytokine tumor necrosis factor (TNF). To address the function of TNF from this cellular source in focal cerebral ischemia we used TNF conditional knock out mice (LysMcreTNF fl/fl) in which the TNF gene was deleted in cells of the myeloid lineage, including microglia. The deletion reduced secreted TNF levels in lipopolysaccharide-stimulated cultured primary microglia by ∼93%. Furthermore, phosphorylated-ERK/ERK ratios were significantly decreased in naïve LysMcreTNF fl/fl mice demonstrating altered ERK signal transduction. Micro-PET using 18 [F]-fluorodeoxyglucose immediately after focal cerebral ischemia showed increased glucose uptake in LysMcreTNF fl/fl mice, representing significant metabolic changes, that translated into increased infarct volumes at 24 hours and 5 days compared to littermates (TNFfl/fl). In naïve LysMcreTNF fl/fl mice cytokine levels were low and comparable to littermates. At 6 hours, TNF producing microglia were reduced by 56% in the ischemic cortex in LysMcreTNF fl/fl mice compared to littermate mice, whereas no TNF + leukocytes were detected. At 24 hours, pro-inflammatory cytokine (TNF, IL-1β, IL-6, IL-5 and CXCL1) levels were significantly lower in LysMcreTNF fl/fl mice, despite comparable infiltrating leukocyte populations. Our results identify microglial TNF as beneficial and neuroprotective in the acute phase and as a modulator of neuroinflammation at later time points after experimental ischemia, which may contribute to regenerative recovery.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Degn, MatildaCopenhagen University Hospital
(författare)
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Sivasaravanaparan, MithulaUniversity of Southern Denmark
(författare)
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Fogtmann, TorbenUniversity of Southern Denmark
(författare)
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Andersen, Maria GammelstrupUniversity of Southern Denmark
(författare)
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Trojanowsky, Michelle D.University of Miami
(författare)
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Gao, HanUniversity of Miami
(författare)
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Hvidsten, SvendOdense University Hospital
(författare)
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Baun, ChristinaOdense University Hospital
(författare)
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Deierborg, TomasLund University,Lunds universitet,Neuroinflammation,Forskargrupper vid Lunds universitet,Lund University Research Groups(Swepub:lu)expb-tol
(författare)
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Finsen, BenteUniversity of Southern Denmark
(författare)
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Kristensen, Bjarne WintherOdense University Hospital,University of Southern Denmark
(författare)
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Bak, Sara ThornbyUniversity of Southern Denmark
(författare)
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Meyer, MortenUniversity of Southern Denmark
(författare)
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Lee, JaeUniversity of Miami
(författare)
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Nedospasov, Sergei A.P.N. Lebedev Physical Institute of the Russian Academy of Sciences,Lomonosov Moscow State University
(författare)
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Brambilla, RobertaUniversity of Miami
(författare)
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Lambertsen, Kate LykkeUniversity of Southern Denmark,Odense University Hospital
(författare)
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University of Southern DenmarkCopenhagen University Hospital
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Scientific Reports: Springer Science and Business Media LLC62045-2322
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Till lärosätets databas
- Av författaren/redakt...
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Clausen, Bettina ...
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Degn, Matilda
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Sivasaravanapara ...
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Fogtmann, Torben
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Andersen, Maria ...
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Trojanowsky, Mic ...
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visa fler...
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Gao, Han
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Hvidsten, Svend
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Baun, Christina
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Deierborg, Tomas
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Finsen, Bente
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Kristensen, Bjar ...
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Bak, Sara Thornb ...
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Meyer, Morten
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Lee, Jae
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Nedospasov, Serg ...
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Brambilla, Rober ...
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Lambertsen, Kate ...
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Neurologi
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Scientific Repor ...
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Lunds universitet