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DNA-launched RNA re...
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Szurgot, IKarolinska Institutet
(författare)
DNA-launched RNA replicon vaccines induce potent anti-SARS-CoV-2 immune responses in mice
- Artikel/kapitelEngelska2021
Förlag, utgivningsår, omfång ...
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2021-02-04
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Springer Science and Business Media LLC,2021
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LIBRIS-ID:oai:prod.swepub.kib.ki.se:146104530
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http://kipublications.ki.se/Default.aspx?queryparsed=id:146104530URI
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https://doi.org/10.1038/s41598-021-82498-5DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
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The outbreak of the SARS-CoV-2 virus and its rapid spread into a global pandemic made the urgent development of scalable vaccines to prevent coronavirus disease (COVID-19) a global health and economic imperative. Here, we characterized and compared the immunogenicity of two alphavirus-based DNA-launched self-replicating (DREP) vaccine candidates encoding either SARS-CoV-2 spike glycoprotein (DREP-S) or a spike ectodomain trimer stabilized in prefusion conformation (DREP-Secto). We observed that the two DREP constructs were immunogenic in mice inducing both binding and neutralizing antibodies as well as T cell responses. Interestingly, the DREP coding for the unmodified spike turned out to be more potent vaccine candidate, eliciting high titers of SARS-CoV-2 specific IgG antibodies that were able to efficiently neutralize pseudotyped virus after a single immunization. In addition, both DREP constructs were able to efficiently prime responses that could be boosted with a heterologous spike protein immunization. These data provide important novel insights into SARS-CoV-2 vaccine design using a rapid response DNA vaccine platform. Moreover, they encourage the use of mixed vaccine modalities as a strategy to combat SARS-CoV-2.
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Hanke, LKarolinska Institutet
(författare)
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Sheward, DJKarolinska Institutet
(författare)
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Vidakovics, LPKarolinska Institutet
(författare)
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Murrell, BKarolinska Institutet
(författare)
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McInerney, GMKarolinska Institutet
(författare)
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Liljestrom, P
(författare)
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Karolinska Institutet
(creator_code:org_t)
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Ingår i:Scientific reports: Springer Science and Business Media LLC11:1, s. 3125-2045-2322
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