onr:"swepub:oai:prod.swepub.kib.ki.se:1943598"
Sökning: onr:"swepub:oai:prod.swepub.kib.ki.se:1943598" > Transcriptional ter...
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 02616naa a2200289 4500 | |
| 001 | oai:prod.swepub.kib.ki.se:1943598 | |
| 003 | SwePub | |
| 008 | 240701s1998 | |||||||||||000 ||eng| | |
| 024 | 7 | a http://kipublications.ki.se/Default.aspx?queryparsed=id:19435982 URI |
| 024 | 7 | a https://doi.org/10.1101/gad.12.17.27592 DOI |
| 040 | a (SwePub)ki | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Bauren, G4 aut |
| 245 | 1 0 | a Transcriptional termination in the Balbiani ring 1 gene is closely coupled to 3'-end formation and excision of the 3'-terminal intron |
| 264 | c 1998-09-01 | |
| 264 | 1 | b Cold Spring Harbor Laboratory,c 1998 |
| 520 | a We have analyzed transcription termination, 3′-end formation, and excision of the 3′-terminal intron in vivo in the Balbiani ring 1 (BR1) gene and its pre-mRNA. We show that full-length RNA transcripts are evenly spaced on the gene from a position 300 bp upstream to a region 500–700 bp downstream of the polyadenylation sequence. Very few full-length transcripts and no short, cleaved, nascent transcripts could be observed downstream of this region. Pre-mRNA with 10–20 adenylate residues accumulates at the active gene and then rapidly leaves from the gene locus. Only polyadenylated pre-mRNAs could be detected in the nucleoplasm. Our results are consistent with the hypothesis that transcription termination occurs in a narrow region for the majority of transcripts, simultaneous with 3′-end formation. Excision of the 3′-terminal intron occurs before 3′-end formation in about 5% of the nascent transcripts. When transcription terminates, 3′ cleavage takes place and 10–20 adenylate residues are added, the 3′-terminal intron is excised from additionally about 75% of the pre-mRNA at the gene locus. Our data support a close temporal and spatial coupling of transcription termination and the cleavage and initial polyadenylation of 3′-end formation. Excision of the 3′-terminal intron is highly stimulated as the cleavage/polyadenylation complex assembles and 3′-end formation is initiated. | |
| 700 | 1 | a Belikov, Su Karolinska Institutet4 aut |
| 700 | 1 | a Wieslander, L4 aut |
| 710 | 2 | a Karolinska Institutet4 org |
| 773 | 0 | t Genes & developmentd : Cold Spring Harbor Laboratoryg 12:17, s. 2759-2769q 12:17<2759-2769x 0890-9369x 1549-5477 |
| 856 | 4 | u http://genesdev.cshlp.org/content/12/17/2759.full.pdf |
| 856 | 4 8 | u http://kipublications.ki.se/Default.aspx?queryparsed=id:1943598 |
| 856 | 4 8 | u https://doi.org/10.1101/gad.12.17.2759 |
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