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Cell-Based Assay To...
Cell-Based Assay To Quantify the Antioxidant Effect of Food-Derived Carotenoids Enriched in Postprandial Human Chylomicrons
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- Sanchez-Campillo, M. (author)
- Universidad de Murcia,University of Murcia
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- Perez-Llamas, F. (author)
- Universidad de Murcia,University of Murcia
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- Gonzalez-Silvera, D. (author)
- Universidad de Murcia,University of Murcia
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- Martinez-Tomas, R. (author)
- Universidad de Murcia,University of Murcia
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- Burgos, M. I. (author)
- Hospital Virgen de la Arrixaca
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- Wellner, Anna, 1982 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Aviles, F. (author)
- Hospital Virgen de la Arrixaca
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- Parra, S. (author)
- Hospital Virgen de la Arrixaca
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- Bialek, L (author)
- Unilever
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- Alminger, Marie, 1957 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Larque, E. (author)
- Universidad de Murcia,University of Murcia
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(creator_code:org_t)
- 2010-10-06
- 2010
- English.
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In: Journal of Agricultural and Food Chemistry. - : American Chemical Society (ACS). - 0021-8561 .- 1520-5118. ; 58:20, s. 10864-10868
- Related links:
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http://dx.doi.org/10...
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https://doi.org/10.1...
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Abstract
Subject headings
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- We developed a new method to evaluate the antioxidant effect of food products in a biological system. The antioxidant status of HepG2 cells was quantified after incubation with postprandial human chylomicrons after the intake of vegetable products. Three subjects consumed in a meal a vegetable soup containing 8.4 mg of beta-carotene and 9 mg of lycopene. After 5 h, the subjects consumed a second meal without carotenoids. Blood samples were collected at basal time and every hour for 9 h. Chylomicrons were isolated from serum samples and used for both carotenoid quantification and HepG2 stimulation. Carotenoid in chylomicrons followed an inter-individual and bimodal carotenoid response. We demonstrated the antioxidant effect of postprandial chylomicrons in HepG2 at the time of maximum carotenoid concentration of chylomicrons with respect to basal time. This cell-based assay seems to be a useful method to evaluate the antioxidant effect of fruit and vegetable products in a biological system.
Subject headings
- LANTBRUKSVETENSKAPER -- Lantbruksvetenskap, skogsbruk och fiske -- Livsmedelsvetenskap (hsv//swe)
- AGRICULTURAL SCIENCES -- Agriculture, Forestry and Fisheries -- Food Science (hsv//eng)
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Keyword
- HepG2 cells
- dietary
- beta-carotene
- salmonella-typhimurium
- Reactive oxygen species
- tomato lycopene
- postprandial
- lycopene
- remnants
- hepg2 cells
- chylomicron
- beta-carotene
- oxidative stress
- vegetable and fruit food
- metabolism
- bioavailability
- plasma
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Sanchez-Campillo ...
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Perez-Llamas, F.
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Gonzalez-Silvera ...
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Martinez-Tomas, ...
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Burgos, M. I.
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Wellner, Anna, 1 ...
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show more...
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Aviles, F.
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Parra, S.
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Bialek, L
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Alminger, Marie, ...
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Larque, E.
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show less...
- About the subject
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- AGRICULTURAL SCIENCES
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AGRICULTURAL SCI ...
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and Agriculture Fore ...
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and Food Science
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
- Articles in the publication
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Journal of Agric ...
- By the university
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Chalmers University of Technology