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Cubosomes post-load...
Cubosomes post-loaded with antimicrobial peptides: Characterization, bactericidal effect and proteolytic stability
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- Boge, Lukas, 1987 (author)
- RISE,Chalmers University of Technology,Research Institutes of Sweden (RISE),Kemi och material
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- Umerska, Anita (author)
- INSERM U 1066, France ; Université Angers, France,National Institute for Health and Medical Research, France
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- Matougui, Nada (author)
- INSERM U 1066, France ; Université Angers,France,National Institute for Health and Medical Research, France
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- Bysell, Helena (author)
- RISE,SP Sveriges Tekniska Forskningsinstitut AB,Research Institutes of Sweden (RISE),Kemi och material
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- Ringstad, Lovisa (author)
- RISE,SP Sveriges Tekniska Forskningsinstitut AB,Research Institutes of Sweden (RISE),Kemi och material
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- Davoudi, Mina (author)
- Lund University,Lunds universitet,Dermatologi och venereologi, Lund,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Dermatology and Venereology (Lund),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine,Lund University, Sweden
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- Eriksson, Jonny (author)
- Uppsala universitet,Uppsala University,Analytisk kemi,Edwards grupp,Uppsala University, Sweden
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- Edwards, Katarina (author)
- Uppsala universitet,Uppsala University,Analytisk kemi,Edwards grupp,Uppsala University, Sweden
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- Andersson, Martin, 1974 (author)
- Chalmers University of Technology,Chalmers University of Technology, Sweden
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(creator_code:org_t)
- Elsevier BV, 2017
- 2017
- English.
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In: International Journal of Pharmaceutics. - : Elsevier BV. - 0378-5173 .- 1873-3476. ; 526:1-2, s. 400-412
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https://doi.org/10.1...
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Abstract
Subject headings
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- Novel antibiotics, such as antimicrobial peptides (AMPs), have recently attended more and more attraction. In this work, dispersed cubic liquid crystalline gel (cubosomes) was used as drug delivery vehicles for three AMPs (AP114, DPK-060 and LL-37). Association of peptides onto cubosomes was studied at two cubosome/peptide ratios using high performance liquid chromatography, ?-potential and circular dichroism measurements. AMPs impact on the cubosome structure was investigated using small angle x-ray scattering and cryogenic transmission electron microscopy. The antimicrobial effect of the AMP loaded cubosomes was studied in vitro by minimum inhibitory concentration and time-kill assays. Proteolytic protection was investigated by incubating the formulations with two elastases and the antimicrobial effect after proteolysis was studied using radial diffusion assay. Different association efficacy onto the cubosomes was observed among the AMPs, with LL-37 showing greatest association (>60%). AP114 loaded cubosomes displayed a preserved antimicrobial effect, whereas for LL-37 the broad spectrum bacterial killing was reduced to only comprise Gram-negative bacteria. Interestingly, DPK-060 loaded cubosomes showed a slight enhanced effect against S. aureus and E. coli strains. Moreover, the cubosomes were found to protect LL-37 from proteolytic degradation, resulting in a significantly better bactericidal effect after being subjected to elastase, compared to unformulated peptide.
Subject headings
- NATURVETENSKAP -- Kemi -- Fysikalisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Physical Chemistry (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmakologi och toxikologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmacology and Toxicology (hsv//eng)
Keyword
- Cubosome
- Amp
- Antimicrobial peptide
- Glycerol monooleate
- Proteolysis
- Liquid crystalline nanoparticle
- Amp
- Antimicrobial peptide
- Cubosome
- Glycerol monooleate
- Liquid crystalline nanoparticle
- Proteolysis
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Boge, Lukas, 198 ...
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Umerska, Anita
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Matougui, Nada
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Bysell, Helena
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Ringstad, Lovisa
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Davoudi, Mina
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show more...
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Eriksson, Jonny
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Edwards, Katarin ...
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Andersson, Marti ...
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
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and Physical Chemist ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Pharmacology and ...
- Articles in the publication
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International Jo ...
- By the university
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Chalmers University of Technology
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Lund University
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Uppsala University
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RISE