Plasmon-Enhanced Colorimetric ELISA with Single Molecule Sensitivity

Chen, Si, 1985 (author): Chalmers tekniska högskola,Chalmers University of Technology

Svedendahl, Mikael, 1984 (author): Chalmers tekniska högskola,Chalmers University of Technology

Käll, Mikael, 1963 (author): Chalmers tekniska högskola,Chalmers University of Technology

(creator_code:org_t)

2011-03-23
2011
English.
In: Nano Letters. - : American Chemical Society (ACS). - 1530-6992 .- 1530-6984. ; 11:4, s. 1826-1830

Related links:: http://dx.doi.org/10...; show more...; https://doi.org/10.1...; https://research.cha...; https://urn.kb.se/re...; show less...

Abstract Subject headings

Robust but ultrasensitive biosensors with a capability of detecting low abundance biomarkers could revolutionize clinical diagnostics and enable early detection of cancer, neurological diseases, and infections. We utilized a combination of localized surface plasmon resonance (LSPR) refractive index sensing and the well-known enzyme-linked immunosorbent assay to develop a simple colorimetric biosensing methodology with single molecule sensitivity. The technique is based on spectral imaging of a large number of isolated gold nanoparticles. Each particle binds a variable number of horseradish peroxidase (HRP) enzyme molecules that catalyze a localized precipitation reaction at the particle surface. The enzymatic reaction dramatically amplifies the shift of the LSPR scattering maximum, lambda(max), and makes it possible to detect the presence of only one or a few HRP molecules per particle.

NATURVETENSKAP -- Fysik (hsv//swe)
NATURAL SCIENCES -- Physical Sciences (hsv//eng)
NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
TEKNIK OCH TEKNOLOGIER -- Nanoteknik (hsv//swe)
ENGINEERING AND TECHNOLOGY -- Nano-technology (hsv//eng)
NATURVETENSKAP -- Fysik -- Annan fysik (hsv//swe)
NATURAL SCIENCES -- Physical Sciences -- Other Physics Topics (hsv//eng)
NATURVETENSKAP -- Fysik -- Atom- och molekylfysik och optik (hsv//swe)
NATURAL SCIENCES -- Physical Sciences -- Atom and Molecular Physics and Optics (hsv//eng)

By the author/editor: Chen, Si, 1985; Svedendahl, Mika ...; Van Duyne, R. P.; Käll, Mikael, 19 ...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biophysics

NATURAL SCIENCES: NATURAL SCIENCES; and Physical Science ...; and Other Physics To ...

NATURAL SCIENCES: NATURAL SCIENCES; and Physical Science ...; and Atom and Molecul ...

By the university: Chalmers University of Technology; Royal Institute of Technology

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.