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FLOW LINEAR DICHROI...
FLOW LINEAR DICHROISM AND ELECTRON-MICROSCOPIC ANALYSIS OF PROTEIN-DNA COMPLEXES OF A MUTANT UVRB PROTEIN THAT BINDS TO BUT CANNOT KINK DNA
- Artikel/kapitelEngelska1994
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LIBRIS-ID:oai:research.chalmers.se:b5ecec64-d51b-4fe4-8660-a3b52d4cdc89
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https://research.chalmers.se/publication/144031URI
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https://doi.org/10.1006/jmbi.1994.1541DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
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Ämneskategori:ref swepub-contenttype
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(A)BC excinuclease of Escherichia coli is the enzymatic activity resulting from sequential and partially overlapping actions of UvrA, UvrB, and UvrC protein. UvrA is a molecular matchmaker which promotes the formation of a stable UvrB-damaged DNA complex in which the DNA is kinked by about 130 degrees. The UvrB-DNA complex is then recognized by UvrC) and two incisions are made in the DNA by the joint actions of UvrC and UvrB. A mutant of UvrB (D478A) can be loaded onto the DNA but it does not interact with UvrC to cause a nick 3' to the lesion. Based on the lack of a DNase-I-hypersensitive site in the footprint of the mutant, it was proposed that the lack of incision was due to the inability of the mutant UvrB to kink the DNA. In the current study we have investigated the interaction of the mutant UvrB with DNA using two biophysical methods, flow linear dichroism and electron microscopy. Both methods reveal that the mutant UvrB is unable to bend DNA.
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Biuppslag (personer, institutioner, konferenser, titlar ...)
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Takahashi, M.
(författare)
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Delagoutte, E.
(författare)
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Bertrandburggraf, E.
(författare)
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Wang, Y. H.
(författare)
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Nordén, Bengt,1945Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)norden
(författare)
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Fuchs, R. P. P.
(författare)
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Griffith, J.
(författare)
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Sancar, A.
(författare)
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Chalmers tekniska högskola
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Journal of Molecular Biology: Elsevier BV241:5, s. 645-6500022-28361089-8638
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