Ten polymorphic microsatellite markers for Hieracium s.s. (Asteraceae)

• Eight novel microsatellite markers were developed for Hieracium s.s. (excluding Pilosella) using a modified FIASCO protocol, and two out of nine microsatellites that were originally developed for Pilosella officinarum were optimised for Hieracium s.s.. All ten microsatellites consistently amplified loci from a wide range of representatives from north and central European species of various sections of the genus. When tested on 19 individuals, nine microsatellites were found to be highly polymorphic with 6–29 alleles, while one microsatellite marker was monomorphic. Cross-amplification showed that six of the ten microsatellites also amplified products in one taxon of Pilosella, P. floribunda × officinarum.

Subject headings

NATURVETENSKAP  -- Biologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences (hsv//eng)

NATURVETENSKAP  -- Biologi -- Botanik (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Botany (hsv//eng)

Keyword

microsatellite marker

FIASCO

Hieracium

Pilosella

genetic variation

cross-amplification

Publication and Content Type

art (subject category)

ref (subject category)