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LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00005754naa a2200541 4500
001oai:DiVA.org:oru-94361
003SwePub
008210916s2022 | |||||||||||000 ||eng|
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-943612 URI
0247 a https://doi.org/10.1136/sextrans-2021-0549882 DOI
040 a (SwePub)oru
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Cole, Michelle Jayneu National Infection Service, Public Health England, London, UK4 aut
2451 0a No widespread dissemination of Chlamydia trachomatis diagnostic :b escape variants and the impact of Neisseria gonorrhoeae positivity on the Aptima Combo 2 assay
264 c 2021-09-11
264 1b BMJ Publishing Group Ltd,c 2022
338 a print2 rdacarrier
500 a Funding agency:NIHR Health Protection Research Unit in Behavioural Science and Evaluation at the University of Bristol
520 a OBJECTIVES: (NG) with the Hologic Aptima CT (ACT) assay was recommended to identify any CT variants.METHODS: From June to October 2019, specimens with discrepant AC2/ACT CT results were submitted to Public Health England and screened for detectable CT DNA using an inhouse real-time (RT)-PCR. When enough DNA was present, partial CT 23S rRNA gene sequencing was performed. Analysis of available relative light units and interpretative data was performed.RESULTS: A total of 317 discordant AC2/ACT specimens were collected from 315 patients. Three hundred were tested on the RT-PCR; 53.3% (n=160) were negative and 46.7% (n=140) were positive. Due to low DNA load in most specimens, sequencing was successful for only 36 specimens. The CT 23S rRNA wild-type sequence was present in 32 specimens, and two variants with C1514T or G1523A mutation were detected in four specimens from three patients. Of the discordant specimens with NG interpretation, 36.6% of NG-negative/CT-negative AC2 specimens had detectable CT DNA on the inhouse RT-PCR vs 53.3% of NG-positive/CT-negative specimens.CONCLUSIONS: No widespread dissemination of AC2 diagnostic-escape CT variants has occurred in England. We however identified the impact of NG positivity on the discordant AC2/ACT specimens; a proportion appeared due to NG positivity and the associated NG signal, rather than any diagnostic-escape variants or low DNA load. Several patients with gonorrhoea may therefore receive false-negative AC2 CT results. Single diagnostic targets and multiplex diagnostic assays have their limitations such as providing selection pressure for escape mutants and potentially reduced sensitivity, respectively. These limitations must be considered when establishing diagnostic pathways.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Infektionsmedicin0 (SwePub)302092 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Infectious Medicine0 (SwePub)302092 hsv//eng
653 a Chlamydia trachomatis
653 a Neisseria gonorrhoeae
653 a chlamydia infections
653 a molecular diagnostic techniques
653 a nucleic acid amplification techniques
7001 a Davis, Grahame S.u National Infection Service, Public Health England, London, UK4 aut
7001 a Fifer, Helenu National Infection Service, Public Health England, London, UK4 aut
7001 a Saunders, John Michaelu National Infection Service, Public Health England, London, UK; Research Department of Infection and Population Health, University College London, London, UK4 aut
7001 a Unemo, Magnus,d 1970-u Örebro universitet,Institutionen för medicinska vetenskaper,Region Örebro län,WHO Collaborating Centre for Gonorrhoea and Other STIs, National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Microbiology4 aut0 (Swepub:oru)muo
7001 a Hadad, Ronza,d 1984-u Örebro universitet,Institutionen för medicinska vetenskaper4 aut0 (Swepub:oru)rahd
7001 a Roberts, David J.u National Infection Service, Public Health England, London, UK4 aut
7001 a Fazal, Mohammedu National Infection Service, Public Health England, London, UK4 aut
7001 a Day, Michaela Joanneu National Infection Service, Public Health England, London, UK4 aut
7001 a Minshull, Jacku National Infection Service, Public Health England, London, UK4 aut
7001 a Muir, Peteru Bristol Public Health Laboratory, Public Health England, Bristol, UK4 aut
7001 a Horner, Paddy J.u School of Social and Community Medicine, University of Bristol, Bristol, UK; Bristol Sexual Health Centre, University Hospitals Bristol NHS Foundation Trust, Bristol, UK4 aut
7001 a Gill, Noel O.u National Infection Service, Public Health England, London, UK4 aut
7001 a Folkard, Kateu National Infection Service, Public Health England, London, UK4 aut
7102 a National Infection Service, Public Health England, London, UKb National Infection Service, Public Health England, London, UK; Research Department of Infection and Population Health, University College London, London, UK4 org
7730 t Sexually Transmitted Infectionsd : BMJ Publishing Group Ltdg 98:5, s. 366-370q 98:5<366-370x 1368-4973x 1472-3263
8564 u https://doi.org/10.1136/sextrans-2021-054988y Fulltext
8564 u https://discovery.ucl.ac.uk/10137437/3/Saunders_No%20widespread%20dissemination%20of%20Chlamydia%20trachomatis%20diagnostic-escape%20variants%20and%20the%20impact%20of%20Neisseria%20gonorrhoeae%20positivity%20on%20the%20Aptima%20Combo%202%20assay_AAM.pdf
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-94361
8564 8u https://doi.org/10.1136/sextrans-2021-054988

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