Sökning: (WFRF:(Tolmachev Vladimir Professor)) >
Phase I study of 99...
Phase I study of 99mTc-ADAPT6, a scaffold protein-based probe for visualization of HER2 expression in breast cancer
-
- Bragina, Olga (författare)
- Russian Acad Sci, Tomsk Natl Res Med Ctr, Canc Res Inst, Dept Nucl Med, Tomsk, Russia.;Tomsk Polytech Univ, Res Ctr Oncotheranost, Res Sch Chem & Appl Biomed Sci, Tomsk, Russia.
-
- von Witting, Emma (författare)
- KTH,Proteinteknologi
-
- Garousi, Javad (författare)
- Uppsala universitet,Medicinsk strålningsvetenskap,Uppsala Univ, Dept Immunol Genet & Pathol, SE-75181 Uppsala, Sweden.
-
visa fler...
-
- Zelchan, Roman (författare)
- Russian Acad Sci, Tomsk Natl Res Med Ctr, Canc Res Inst, Dept Nucl Med, Tomsk, Russia.;Tomsk Polytech Univ, Res Ctr Oncotheranost, Res Sch Chem & Appl Biomed Sci, Tomsk, Russia.
-
- Sandström, Mattias (författare)
- Uppsala universitet,Radiologi,Uppsala Univ, Dept Surg Sci, Radiol & Nucl Med, Uppsala, Sweden.;Uppsala Univ Hosp, Med Phys, Uppsala, Sweden.
-
- Orlova, Anna, 1960- (författare)
- Uppsala universitet,Theranostics,Tomsk Polytechnic University,Tomsk Polytech Univ, Res Ctr Oncotheranost, Res Sch Chem & Appl Biomed Sci, Tomsk, Russia.;Uppsala Univ, Dept Med Chem, Uppsala, Sweden.
-
- Medvedeva, Anna (författare)
- Russian Acad Sci, Tomsk Natl Res Med Ctr, Canc Res Inst, Dept Nucl Med, Tomsk, Russia.
-
- Doroshenko, Artem (författare)
- Russian Acad Sci, Tomsk Natl Res Med Ctr, Canc Res Inst, Dept Gen Oncol, Tomsk, Russia.
-
- Vorobyeva, Anzhelika (författare)
- Uppsala universitet,Medicinsk strålningsvetenskap,Tomsk Polytechnic University,Tomsk Polytech Univ, Res Ctr Oncotheranost, Res Sch Chem & Appl Biomed Sci, Tomsk, Russia.;Uppsala Univ, Dept Immunol Genet & Pathol, SE-75181 Uppsala, Sweden.
-
- Lindbo, Sarah (författare)
- KTH,Proteinteknologi
-
- Borin, Jesper (författare)
- KTH,Proteinteknologi
-
- Tarabanovskaya, Natalya (författare)
- Russian Acad Sci, Tomsk Natl Res Med Ctr, Canc Res Inst, Dept Gen Oncol, Tomsk, Russia.
-
- Sörensen, Jens (författare)
- Uppsala universitet,Radiologi,Uppsala Univ, Dept Surg Sci, Radiol & Nucl Med, Uppsala, Sweden.
-
- Hober, Sophia, Professor, 1965- (författare)
- KTH,Centrum för Bioprocessteknik, CBioPT,Science for Life Laboratory, SciLifeLab,Albanova VinnExcellence Center for Protein Technology, ProNova,Proteinteknologi
-
- Chernov, Vladimir (författare)
- Russian Acad Sci, Tomsk Natl Res Med Ctr, Canc Res Inst, Dept Nucl Med, Tomsk, Russia.;Tomsk Polytech Univ, Res Ctr Oncotheranost, Res Sch Chem & Appl Biomed Sci, Tomsk, Russia.
-
- Tolmachev, Vladimir (författare)
- Uppsala universitet,Medicinsk strålningsvetenskap,Tomsk Polytechnic University,Tomsk Polytech Univ, Res Ctr Oncotheranost, Res Sch Chem & Appl Biomed Sci, Tomsk, Russia.;Uppsala Univ, Dept Immunol Genet & Pathol, SE-75181 Uppsala, Sweden.
-
visa färre...
-
Russian Acad Sci, Tomsk Natl Res Med Ctr, Canc Res Inst, Dept Nucl Med, Tomsk, Russia;Tomsk Polytech Univ, Res Ctr Oncotheranost, Res Sch Chem & Appl Biomed Sci, Tomsk, Russia. Proteinteknologi (creator_code:org_t)
- 2020-08-17
- 2021
- Engelska.
-
Ingår i: Journal of Nuclear Medicine. - : Society of Nuclear Medicine. - 0161-5505 .- 1535-5667 .- 2159-662X. ; 62:4, s. 493-499
- Relaterad länk:
-
https://jnm.snmjourn...
-
visa fler...
-
https://urn.kb.se/re...
-
https://doi.org/10.2...
-
https://urn.kb.se/re...
-
visa färre...
Abstract
Ämnesord
Stäng
- Radionuclide molecular imaging of human epidermal growth factor (HER2) expression may be helpful to stratify breast and gastroesophageal cancer patients for HER2-targeting therapies. ADAPTs (albumin-binding domain derived affinity proteins) are a new type of small (46-59 amino acids) proteins useful as probes for molecular imaging. The aim of this first-in-human study was to evaluate biodistribution, dosimetry, and safety of the HER2-specific 99mTc-ADAPT6.METHODS: Twenty-nine patients with primary breast cancerwere included. In 22 patients with HER2-positive (n = 11) or HER2-negative (n = 11) histopathology an intravenous injection with 385±125 MBq 99mTc-ADAPT6 was performed, randomized to an injected protein mass of either 500 µg (n = 11) or 1000 µg (n = 11). Planar scintigraphy followed by SPECT imaging was performed after 2, 4, 6 and 24 h. An additional cohort (n = 7) was injected with 165±29 MBq (injected protein mass 250 µg) and imaging was performed after 2 h only.RESULTS: Injections of 99mTc-ADAPT6 at all injected mass levels were well tolerated and not associated with adverse effects. 99mTc-ADAPT6 cleared rapidly from blood and most other tissues. The normal organs with the highest accumulation were kidney, liver and lung. Effective doses were 0.009±0.002 and 0.010±0.003 mSv/MBq for injected protein masses of 500 and 1000 µg, respectively. Injection of 500 µg resulted in excellent discrimination between HER2-positive and HER2-negative tumors already 2 h after injection (tumor-to-contralateral breast ratio was 37±19 vs 5±2, p<0.01). The tumor-to-contralateral breast ratios for HER2-positive tumors were significantly (p<0.05) higher for injected mass of 500 µg than for both 250 and 1000 µg.CONCLUSION: Injections of 99mTc-ADAPT6 are safe and associated with low absorbed and effective doses. Protein dose of 500 µg is preferable for discrimination between tumors with high and low expression of HER2. Further studies are justified to evaluate if 99mTc-ADAPT6 can be used as an imaging probe for stratification of patients for HER2-targeting therapy in the areas where PET imaging is not readily available.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Radiologi och bildbehandling (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Radiology, Nuclear Medicine and Medical Imaging (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Nyckelord
- 99mTc
- ADAPT6
- HER2
- Molecular Imaging
- Oncology: Breast
- Phase I
- SPECT
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Bragina, Olga
-
von Witting, Emm ...
-
Garousi, Javad
-
Zelchan, Roman
-
Sandström, Matti ...
-
Orlova, Anna, 19 ...
-
visa fler...
-
Medvedeva, Anna
-
Doroshenko, Arte ...
-
Vorobyeva, Anzhe ...
-
Lindbo, Sarah
-
Borin, Jesper
-
Tarabanovskaya, ...
-
Sörensen, Jens
-
Hober, Sophia, P ...
-
Chernov, Vladimi ...
-
Tolmachev, Vladi ...
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Klinisk medicin
-
och Radiologi och bi ...
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Klinisk medicin
-
och Cancer och onkol ...
- Artiklar i publikationen
-
Journal of Nucle ...
- Av lärosätet
-
Uppsala universitet
-
Kungliga Tekniska Högskolan