Characterisation of receptors for IGF-I and insulin; evidence for hybrid insulin/IGF-I receptor in human coronary artery endothelial cells

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Fältnamn	Indikatorer	Metadata
000		04534naa a2200409 4500
001		oai:lup.lub.lu.se:7fdd0868-56e2-421f-b062-235a9f202635
003		SwePub
008		160401s2006 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
009		oai:DiVA.org:liu-12555
024	7	a https://lup.lub.lu.se/record/11373652 URI
024	7	a https://doi.org/10.1016/j.ghir.2006.06.0032 DOI
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-125552 URI
040		a (SwePub)lud (SwePub)liu
041		a engb eng
042		9 SwePub
072	7	a art2 swepub-publicationtype
072	7	a ref2 swepub-contenttype
100	1	a Chisalita, Simona I.,d 1972-u Linköpings universitet,Cellbiologi,Hälsouniversitetet4 aut0 (Swepub:liu)ioach17
245	1 0	a Characterisation of receptors for IGF-I and insulin; evidence for hybrid insulin/IGF-I receptor in human coronary artery endothelial cells
264	1	b Elsevier BV,c 2006
520		a OBJECTIVE: Coronary artery disease is a prevalent cause of morbidity and mortality in diabetes. Little is known about insulin-like growth factor-I receptors (IGF-IR) and insulin receptors (IR) in human coronary endothelium. Our aim was to characterize IGF-IR and IR in human coronary artery endothelial cells (HCAEC). DESIGN: Cultured human coronary artery endothelial cells were used. Gene expression was measured by quantitative real-time RT-PCR analysis and receptor affinity by ligand binding. Receptor protein, phosphorylation of IGF-IR and IR beta-subunit as well as the presence of hybrid insulin receptor/Insulin-like growth factor-I receptor (Hybrid IR/IGF-IR) was analyzed by immunoprecipitation and Western blot. Postreceptor effects of insulin and IGF-I were assed by (3)H-thymidine incorporation. RESULTS: The gene expression of IGF-IR was several folds higher than that of IR. and insulin receptor isoform A (IR-A) was 20-fold more expressed than insulin receptor isoform B (IR-B) in HCAEC. The specific binding of (125)I-IGF-I was higher than that of (125)I-insulin. Insulin and the new long acting insulin analog, glargine, interacted with the IGF-IR with over thousand and 100-fold less potency than IGF-I itself, whereas IGF-II had 6 times lower potency than IGF-I. Phosphorylation of the IGF-IR beta-subunit was obtained by concentrations of 10(-10)-10(-8)M IGF-I, 10(-6)M of insulin, inconsistently by 10(-8)M insulin and not at all by 10(-10)-10(-9)M insulin. The IR beta-subunit was phosphorylated by insulin and IGF-I at concentrations of 10(-9)-10(-8)M. When immunoprecipitating with specific monoclonal anti-IR or anti-IGF-IR alpha-subunit antibodies we found bands situated in slightly different positions suggesting the presence of Hybrid IR/IGF-IR. IGF-I, IGF-II and insulin (10(-9)-10(-7)M) had no significant effect on (3)H-thymidine incorporation into DNA. CONCLUSIONS: Human coronary endothelial cells express more IGF-IR than IR, mainly IR-A, and also Hybrid IR/IGF-IR. Both IGF-I and insulin phosphorylate their receptors, but only IGF-I seems to phosphorylate Hybrid IR/IGF-IR. Our study provides experimental evidence for a possible role of IGF-IR, IR and Hybrid IR/IGF-IR in human coronary artery endothelial cells.
650	7	a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Endokrinologi och diabetes0 (SwePub)302052 hsv//swe
650	7	a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Endocrinology and Diabetes0 (SwePub)302052 hsv//eng
653		a Human endothelial cells
653		a Insulin-like growth factor-I
653		a Insulin
653		a Human endothelial cells; Insulin-like growth factor-I; Insulin
653		a MEDICINE
700	1	a Dekker Nitert, Marloesu Linköpings universitet,Lund University,Lunds universitet,Institutionen för experimentell medicinsk vetenskap,Medicinska fakulteten,Department of Experimental Medical Science,Faculty of Medicine,Cellbiologi,Hälsouniversitetet4 aut0 (Swepub:liu)marde07
700	1	a Arnqvist, Hans J.u Linköpings universitet,Cellbiologi,Hälsouniversitetet4 aut0 (Swepub:liu)hanar64
710	2	a Linköpings universitetb Cellbiologi4 org
773	0	t Growth Hormone & Igf Researchd : Elsevier BVg 16:4, s. 258-266q 16:4<258-266x 1532-2238x 1096-6374
856	4	u http://dx.doi.org/10.1016/j.ghir.2006.06.003y FULLTEXT
856	4	u http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-12558y Link to Ph.D. thesis
856	4 8	u https://lup.lub.lu.se/record/1137365
856	4 8	u https://doi.org/10.1016/j.ghir.2006.06.003
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-12555

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