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Evaluation of 92 cardiovascular proteins in dried blood spots collected under field-conditions : Off-the-shelf affinity-based multiplexed assays work well, allowing for simplified sample collection

Broberg, Karin (author)
Karolinska Institutet,Karolinska Institute
Grahn, Karin (author)
Karolinska Institutet,Karolinska Institute
Assarsson, Eva (author)
Lund University,Lunds universitet,Avdelningen för arbets- och miljömedicin,Institutionen för laboratoriemedicin,Medicinska fakulteten,Genetisk arbets- och miljömedicin,Forskargrupper vid Lunds universitet,Division of Occupational and Environmental Medicine, Lund University,Department of Laboratory Medicine,Faculty of Medicine,Genetic Occupational and Environmental Medicine,Lund University Research Groups
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Åberg, Mikael (author)
Uppsala University
Selander, Jenny (author)
Karolinska Institutet,Karolinska Institute
Enroth, Stefan, 1976- (author)
Uppsala universitet,Uppsala University,Science for Life Laboratory, SciLifeLab,Medicinsk genetik och genomik,Institutionen för cell- och molekylärbiologi,Gyllensten
Svensson, Johanna (author)
Uppsala universitet,Uppsala University,Science for Life Laboratory (SciLifeLab),Uppsala kliniska forskningscentrum (UCR)
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 (creator_code:org_t)
2021-02-15
2021
English.
In: BioEssays. - : Wiley. - 0265-9247 .- 1521-1878. ; 43:9
  • Journal article (peer-reviewed)
Abstract Subject headings
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  • Workplace-collected blood spots deposited on filter paper were analysed with multiplexed affinity-based protein assays and found to be suitable for proteomics analysis. The protein extension assay (PEA) was used to characterize 92 proteins using 1.2 mm punches in repeated samples collected from 20 workers. Overall, 97.8% of the samples and 91.3% of the analysed proteins passed quality control. Both within and between spot correlations using six replicates from the same individual were above 0.99, suggesting that comparable levels are obtained from multiple punches from the same spot and from consecutive spots. Protein levels from dried blood and wet serum from the same individuals were compared and the majority of the analysed proteins were found to be significantly correlated. These results open up for simplified sample collection of blood in field conditions for proteomic analysis, but also highlight that not all proteins can be robustly measured from dried whole blood.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)

Keyword

affinity based proteomics
dried blood spots
sample collection
affinity based proteomics

Publication and Content Type

art (subject category)
ref (subject category)

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