Sökning: WFRF:(Hansson Erik) > A new method for is...
Fältnamn | Indikatorer | Metadata |
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000 | 02425naa a2200337 4500 | |
001 | oai:DiVA.org:umu-6863 | |
003 | SwePub | |
008 | 071220s2007 | |||||||||||000 ||eng| | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-68632 URI |
024 | 7 | a https://doi.org/10.1016/j.plaphy.2007.09.0012 DOI |
040 | a (SwePub)umu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Gough, Simon P4 aut |
245 | 1 0 | a A new method for isolating physiologically active Mg-protoporphyrin monomethyl ester, the substrate of the cyclase enzyme of the chlorophyll biosynthetic pathway. |
264 | 1 | b Elsevier BV,c 2007 |
338 | a print2 rdacarrier | |
520 | a Mg-protoporphyrin monomethyl ester (MPE) is a biosynthetic intermediate of chlorophyll and converted by MPE cyclase to protochlorophyllide. Limited availability of MPE has so far hampered cyclase research. In a new, simplified, method MPE was prepared from freeze dried bchE mutant Rhodobacter capsulatus DB575 cells by extraction with acetone/H2O/25% NH3. Isolated MPE was identified by absorption and fluorescence spectroscopy, and its purity was analyzed by HPLC. The extracted MPE was dried and redissolved in buffered DMSO and its substrate activity is shown by enzymatic cyclase assays. A linear time course was observed for MPE conversion to protochlorophyllide by enzymes from barley etioplasts. Our innovation of freeze drying the R. capsulatus cells before extraction provides a high yield method for MPE, which is significantly faster and more reproducible than previous extraction methods. | |
700 | 1 | a Rzeznicka, Kamila4 aut |
700 | 1 | a Peterson Wulff, Ragna4 aut |
700 | 1 | a Francisco, Jose da Cruz4 aut |
700 | 1 | a Hansson, Andreasu Umeå universitet,Institutionen för fysiologisk botanik,Umeå Plant Science Centre (UPSC)4 aut0 (Swepub:umu)anha0063 |
700 | 1 | a Jensen, Poul Erik4 aut |
700 | 1 | a Hansson, Mats4 aut |
710 | 2 | a Umeå universitetb Institutionen för fysiologisk botanik4 org |
773 | 0 | t Plant Physiology and Biochemistryd : Elsevier BVg 45:12, s. 932-6q 45:12<932-6x 0981-9428 |
856 | 4 | u http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Retrieve&list_uids=17949988&dopt=Citation |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-6863 |
856 | 4 8 | u https://doi.org/10.1016/j.plaphy.2007.09.001 |
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