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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 03643naa a2200469 4500 | |
| 001 | oai:research.chalmers.se:3dd1cf03-721e-4fa6-93ae-bac4d74308c6 | |
| 003 | SwePub | |
| 008 | 171007s2013 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://research.chalmers.se/publication/1915832 URI |
| 024 | 7 | a https://doi.org/10.1128/aem.02694-132 DOI |
| 040 | a (SwePub)cth | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a art2 swepub-publicationtype |
| 072 | 7 | a ref2 swepub-contenttype |
| 100 | 1 | a Anfelt, J.u Kungliga Tekniska Högskolan (KTH),Royal Institute of Technology (KTH)4 aut |
| 245 | 1 0 | a Using Transcriptomics To Improve Butanol Tolerance of Synechocystis sp Strain PCC 6803 |
| 264 | 1 | b American Society for Microbiology,c 2013 |
| 520 | a Cyanobacteria are emerging as promising hosts for production of advanced biofuels such as n-butanol and alkanes. However, cyanobacteria suffer from the same product inhibition problems as those that plague other microbial biofuel hosts. High concentrations of butanol severely reduce growth, and even small amounts can negatively affect metabolic processes. An understanding of how cyanobacteria are affected by their biofuel product can enable identification of engineering strategies for improving their tolerance. Here we used transcriptome sequencing (RNA-Seq) to assess the transcriptome response of Synechocystis sp. strain PCC 6803 to two concentrations of exogenous n-butanol. Approximately 80 transcripts were differentially expressed at 40 mg/liter butanol, and 280 transcripts were different at 1 g/liter butanol. Our results suggest a compromised cell membrane, impaired photosynthetic electron transport, and reduced biosynthesis. Accumulation of intracellular reactive oxygen species (ROS) scaled with butanol concentration. Using the physiology and transcriptomics data, we selected several genes for overexpression in an attempt to improve butanol tolerance. We found that overexpression of several proteins, notably, the small heat shock protein HspA, improved tolerance to butanol. Transcriptomics-guided engineering created more solvent-tolerant cyanobacteria strains that could be the foundation for a more productive biofuel host. | |
| 650 | 7 | a NATURVETENSKAPx Biologix Mikrobiologi0 (SwePub)106062 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Microbiology0 (SwePub)106062 hsv//eng |
| 653 | a TOLERANCE | |
| 653 | a SUPEROXIDE-DISMUTASE | |
| 653 | a OXIDATIVE STRESS | |
| 653 | a UV-B | |
| 653 | a PHOTOSYSTEM-II | |
| 653 | a SP PCC-6803 | |
| 653 | a SOLVENT | |
| 653 | a ESCHERICHIA-COLI | |
| 653 | a PROTEOMIC ANALYSIS | |
| 653 | a HYDROGEN-PEROXIDE | |
| 653 | a GENE-EXPRESSION | |
| 700 | 1 | a Hallstrom, B. M.u Novo Nordisk Fonden,Novo Nordisk Foundation4 aut |
| 700 | 1 | a Nielsen, Jens B,d 1962u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)nielsenj |
| 700 | 1 | a Uhlen, M.u Kungliga Tekniska Högskolan (KTH),Royal Institute of Technology (KTH),Novo Nordisk Fonden,Novo Nordisk Foundation4 aut |
| 700 | 1 | a Hudson, E. P.u Kungliga Tekniska Högskolan (KTH),Royal Institute of Technology (KTH)4 aut |
| 710 | 2 | a Kungliga Tekniska Högskolan (KTH)b Novo Nordisk Fonden4 org |
| 773 | 0 | t Applied and Environmental Microbiologyd : American Society for Microbiologyg 79:23, s. 7419-7427q 79:23<7419-7427x 1098-5336x 0099-2240 |
| 856 | 4 | u http://dx.doi.org/10.1128/aem.02694-13y FULLTEXT |
| 856 | 4 | u https://aem.asm.org/content/aem/79/23/7419.full.pdf |
| 856 | 4 8 | u https://research.chalmers.se/publication/191583 |
| 856 | 4 8 | u https://doi.org/10.1128/aem.02694-13 |
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