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Using adjusted loca...
Using adjusted local assortativity with Molecular Pixelation unveils colocalization of membrane proteins with immunological significance
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- Rhomberg-Kauert, Jan (författare)
- Pixelgen Technologies AB, Stockholm, Sweden; Department of Geodesy and Geoinformation, TU Wien, Vienna, Austria
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- Karlsson, Max (författare)
- Pixelgen Technologies AB, Stockholm, Sweden
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- Thiagarajan, Divya (författare)
- Pixelgen Technologies AB, Stockholm, Sweden
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- Kallas, Tomasz (författare)
- Pixelgen Technologies AB, Stockholm, Sweden
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- Karlsson, Filip (författare)
- Pixelgen Technologies AB, Stockholm, Sweden
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- Fredriksson, Simon (författare)
- KTH,Proteinvetenskap,Pixelgen Technologies AB, Stockholm, Sweden
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- Dahlberg, Johan (författare)
- Pixelgen Technologies AB, Stockholm, Sweden
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- Martinez Barrio, Alvaro (författare)
- Pixelgen Technologies AB, Stockholm, Sweden
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(creator_code:org_t)
- Frontiers Media SA, 2024
- 2024
- Engelska.
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Ingår i: Frontiers in Immunology. - : Frontiers Media SA. - 1664-3224. ; 15
- Relaterad länk:
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https://doi.org/10.3...
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https://urn.kb.se/re...
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https://doi.org/10.3...
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Abstract
Ämnesord
Stäng
- Advances in spatial proteomics and protein colocalization are a driving force in the understanding of cellular mechanisms and their influence on biological processes. New methods in the field of spatial proteomics call for the development of algorithms and open up new avenues of research. The newly introduced Molecular Pixelation (MPX) provides spatial information on surface proteins and their relationship with each other in single cells. This allows for in silico representation of neighborhoods of membrane proteins as graphs. In order to analyze this new data modality, we adapted local assortativity in networks of MPX single-cell graphs and created a method that is able to capture detailed information on the spatial relationships of proteins. The introduced method can evaluate the pairwise colocalization of proteins and access higher-order similarity to investigate the colocalization of multiple proteins at the same time. We evaluated the method using publicly available MPX datasets where T cells were treated with a chemokine to study uropod formation. We demonstrate that adjusted local assortativity detects the effects of the stimuli at both single- and multiple-marker levels, which enhances our understanding of the uropod formation. We also applied our method to treating cancerous B-cell lines using a therapeutic antibody. With the adjusted local assortativity, we recapitulated the effect of rituximab on the polarity of CD20. Our computational method together with MPX improves our understanding of not only the formation of cell polarity and protein colocalization under stimuli but also advancing the overall insight into immune reaction and reorganization of cell surface proteins, which in turn allows the design of novel therapies. We foresee its applicability to other types of biological spatial data when represented as undirected graphs.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Nyckelord
- graph theory
- local assortativity
- molecular pixelation
- Rituximab
- single cell
- spatial proteomics
- topological data analysis
- uropod formation
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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