Sökning: WFRF:(Koch Manuel) >
The cartilage-speci...
The cartilage-specific lectin C-type lectin domain family 3 member A (CLEC3A) enhances tissue plasminogen activator-mediated plasminogen activation
-
- Lau, Daniela (författare)
- University of Cologne
-
- Elezagic, Dzemal (författare)
- University of Cologne
-
- Hermes, Gabriele (författare)
- University of Cologne
-
visa fler...
-
- Mörgelin, Matthias (författare)
- Lund University,Lunds universitet,Infektionsmedicin,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Infection Medicine (BMC),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine
-
- Wohl, Alexander P. (författare)
- University of Cologne
-
- Koch, Manuel (författare)
- University of Cologne
-
- Hartmann, Ursula (författare)
- University of Cologne
-
- Höllriegl, Stefan (författare)
- Sana Dreifaltigkeits-Krankenhaus
-
- Wagener, Raimund (författare)
- University of Cologne
-
- Paulsson, Mats (författare)
- University of Cologne
-
- Streichert, Thomas (författare)
- University of Cologne
-
- Klatt, Andreas R. (författare)
- University of Cologne
-
visa färre...
-
(creator_code:org_t)
- 2018
- 2018
- Engelska 12 s.
-
Ingår i: Journal of Biological Chemistry. - 0021-9258. ; 293:1, s. 203-214
- Relaterad länk:
-
http://dx.doi.org/10...
-
visa fler...
-
https://lup.lub.lu.s...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- C-type lectin domain family 3 member A (CLEC3A) is a poorly characterized protein belonging to the superfamily of C-type lectins. Its closest homologue tetranectin binds to the kringle 4 domain of plasminogen and enhances its association with tissue plasminogen activator (tPA) thereby enhancing plasmin production, but whether CLEC3A contributes to plasminogen activation is unknown. Here, we recombinantly expressed murine and human full-length CLEC3As as well as truncated forms of CLEC3A in HEK-293 Epstein-Barr nuclear antigen (EBNA) cells. We analyzed the structure of recombinant CLEC3A by SDS-PAGE and immunoblot, glycan analysis, matrix-assisted laser desorption ionization time-of-flight mass spectrometry, size-exclusion chromatography, circular dichroism spectroscopy, and electron microscopy; compared the properties of the recombinant protein with those of CLEC3A extracted from cartilage; and investigated its tissue distribution and extracellular assembly by immunohistochemistry and immunofluorescence microscopy. We found that CLEC3A mainly occurs as a monomer, but also forms dimers and trimers, potentially via a coiled-coil α-helix. We also noted that CLEC3A can be modified with chondroitin/dermatan sulfate side chains and tends to oligomerize to form higher aggregates. We show that CLEC3A is present in resting, proliferating, and hypertrophic growth-plate cartilage and assembles into an extended extracellular network in cultures of rat chondrosarcoma cells. Further, we found that CLEC3A specifically binds to plasminogen and enhances tPA-mediated plasminogen activation. In summary, we have determined the structure, tissue distribution, and molecular function of the cartilage-specific lectin CLEC3A and show that CLEC3A binds to plasminogen and participates in tPA-mediated plasminogen activation.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Lau, Daniela
-
Elezagic, Dzemal
-
Hermes, Gabriele
-
Mörgelin, Matthi ...
-
Wohl, Alexander ...
-
Koch, Manuel
-
visa fler...
-
Hartmann, Ursula
-
Höllriegl, Stefa ...
-
Wagener, Raimund
-
Paulsson, Mats
-
Streichert, Thom ...
-
Klatt, Andreas R ...
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Medicinska och f ...
-
och Cell och molekyl ...
- Artiklar i publikationen
-
Journal of Biolo ...
- Av lärosätet
-
Lunds universitet