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WFRF:(Kozlova Elena N.)
 

Sökning: WFRF:(Kozlova Elena N.) > Differentiating neu...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00003934naa a2200481 4500
001oai:DiVA.org:uu-178542
003SwePub
008120731s2012 | |||||||||||000 ||eng|
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-1785422 URI
0247 a https://doi.org/10.1007/s00125-012-2542-02 DOI
040 a (SwePub)uu
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Grouwels, G.4 aut
2451 0a Differentiating neural crest stem cells induce proliferation of cultured rodent islet beta cells
264 c 2012-05-23
264 1b Springer Science and Business Media LLC,c 2012
338 a print2 rdacarrier
500 a De 2 förstaförfattarna delar förstaförfattarskapetDe 2 sistaförfattarna delar sistaförfattarskapet
520 a Aims/hypothesisEfficient stimulation of cycling activity in cultured beta cells would allow the design of new strategies for cell therapy in diabetes. Neural crest stem cells (NCSCs) play a role in beta cell development and maturation and increase the beta cell number in co-transplants. The mechanism behind NCSC-induced beta cell proliferation and the functional capacity of the new beta cells is not known.MethodsWe developed a new in vitro co-culture system that enables the dissection of the elements that control the cellular interactions that lead to NCSC-dependent increase in islet beta cells.ResultsMouse NCSCs were cultured in vitro, first in medium that stimulated their proliferation, then under conditions that supported their differentiation. When mouse islet cells were cultured together with the NCSCs, more than 35% of the beta cells showed cycle activity. This labelling index is more than tenfold higher than control islets cultured without NCSCs. Beta cells that proliferated under these culture conditions were fully glucose responsive in terms of insulin secretion. NCSCs also induced beta cell proliferation in islets isolated from 1-year-old mice, but not in dissociated islet cells isolated from human donor pancreas tissue. To stimulate beta cell proliferation, NCSCs need to be in intimate contact with the beta cells.Conclusions/interpretationCulture of islet cells in contact with NCSCs induces highly efficient beta cell proliferation. The reported culture system is an excellent platform for further dissection of the minimal set of factors needed to drive this process and explore its potential for translation to diabetes therapy.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Endokrinologi och diabetes0 (SwePub)302052 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Endocrinology and Diabetes0 (SwePub)302052 hsv//eng
653 a Beta cell
653 a Cell culture
653 a Diabetes
653 a Neural crest
653 a Proliferation
7001 a Vasylovska, Svitlanau Uppsala universitet,Neuroanatomi4 aut0 (Swepub:uu)sviva947
7001 a Olerud, Johanu Uppsala universitet,Institutionen för neurovetenskap4 aut0 (Swepub:uu)joole425
7001 a Leuckx, G.4 aut
7001 a Ngamjariyawat, Anongnadu Uppsala universitet,Neuroanatomi4 aut0 (Swepub:uu)anong303
7001 a Yuchi, Y.4 aut
7001 a Jansson, Leifu Uppsala universitet,Institutionen för medicinsk cellbiologi4 aut0 (Swepub:uu)leifjans
7001 a Van de Casteele, M.4 aut
7001 a Kozlova, Elena N.u Uppsala universitet,Neuroanatomi4 aut0 (Swepub:uu)elenkozl
7001 a Heimberg, H.4 aut
7102 a Uppsala universitetb Neuroanatomi4 org
7730 t Diabetologiad : Springer Science and Business Media LLCg 55:7, s. 2016-2025q 55:7<2016-2025x 0012-186Xx 1432-0428
8564 u https://link.springer.com/content/pdf/10.1007%2Fs00125-012-2542-0.pdf
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-178542
8564 8u https://doi.org/10.1007/s00125-012-2542-0

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