Sökning: WFRF:(Kozlova Elena N.) > Differentiating neu...
Fältnamn | Indikatorer | Metadata |
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000 | 03934naa a2200481 4500 | |
001 | oai:DiVA.org:uu-178542 | |
003 | SwePub | |
008 | 120731s2012 | |||||||||||000 ||eng| | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-1785422 URI |
024 | 7 | a https://doi.org/10.1007/s00125-012-2542-02 DOI |
040 | a (SwePub)uu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Grouwels, G.4 aut |
245 | 1 0 | a Differentiating neural crest stem cells induce proliferation of cultured rodent islet beta cells |
264 | c 2012-05-23 | |
264 | 1 | b Springer Science and Business Media LLC,c 2012 |
338 | a print2 rdacarrier | |
500 | a De 2 förstaförfattarna delar förstaförfattarskapetDe 2 sistaförfattarna delar sistaförfattarskapet | |
520 | a Aims/hypothesisEfficient stimulation of cycling activity in cultured beta cells would allow the design of new strategies for cell therapy in diabetes. Neural crest stem cells (NCSCs) play a role in beta cell development and maturation and increase the beta cell number in co-transplants. The mechanism behind NCSC-induced beta cell proliferation and the functional capacity of the new beta cells is not known.MethodsWe developed a new in vitro co-culture system that enables the dissection of the elements that control the cellular interactions that lead to NCSC-dependent increase in islet beta cells.ResultsMouse NCSCs were cultured in vitro, first in medium that stimulated their proliferation, then under conditions that supported their differentiation. When mouse islet cells were cultured together with the NCSCs, more than 35% of the beta cells showed cycle activity. This labelling index is more than tenfold higher than control islets cultured without NCSCs. Beta cells that proliferated under these culture conditions were fully glucose responsive in terms of insulin secretion. NCSCs also induced beta cell proliferation in islets isolated from 1-year-old mice, but not in dissociated islet cells isolated from human donor pancreas tissue. To stimulate beta cell proliferation, NCSCs need to be in intimate contact with the beta cells.Conclusions/interpretationCulture of islet cells in contact with NCSCs induces highly efficient beta cell proliferation. The reported culture system is an excellent platform for further dissection of the minimal set of factors needed to drive this process and explore its potential for translation to diabetes therapy. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Endokrinologi och diabetes0 (SwePub)302052 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Endocrinology and Diabetes0 (SwePub)302052 hsv//eng |
653 | a Beta cell | |
653 | a Cell culture | |
653 | a Diabetes | |
653 | a Neural crest | |
653 | a Proliferation | |
700 | 1 | a Vasylovska, Svitlanau Uppsala universitet,Neuroanatomi4 aut0 (Swepub:uu)sviva947 |
700 | 1 | a Olerud, Johanu Uppsala universitet,Institutionen för neurovetenskap4 aut0 (Swepub:uu)joole425 |
700 | 1 | a Leuckx, G.4 aut |
700 | 1 | a Ngamjariyawat, Anongnadu Uppsala universitet,Neuroanatomi4 aut0 (Swepub:uu)anong303 |
700 | 1 | a Yuchi, Y.4 aut |
700 | 1 | a Jansson, Leifu Uppsala universitet,Institutionen för medicinsk cellbiologi4 aut0 (Swepub:uu)leifjans |
700 | 1 | a Van de Casteele, M.4 aut |
700 | 1 | a Kozlova, Elena N.u Uppsala universitet,Neuroanatomi4 aut0 (Swepub:uu)elenkozl |
700 | 1 | a Heimberg, H.4 aut |
710 | 2 | a Uppsala universitetb Neuroanatomi4 org |
773 | 0 | t Diabetologiad : Springer Science and Business Media LLCg 55:7, s. 2016-2025q 55:7<2016-2025x 0012-186Xx 1432-0428 |
856 | 4 | u https://link.springer.com/content/pdf/10.1007%2Fs00125-012-2542-0.pdf |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-178542 |
856 | 4 8 | u https://doi.org/10.1007/s00125-012-2542-0 |
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