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Sökning: onr:"swepub:oai:DiVA.org:su-221411" > Protomers of the gr...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00003120naa a2200421 4500
001oai:DiVA.org:su-221411
003SwePub
008230920s2023 | |||||||||||000 ||eng|
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-2214112 URI
0247 a https://doi.org/10.1039/d3cp02661b2 DOI
040 a (SwePub)su
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Ashworth, Eleanor K.4 aut
2451 0a Protomers of the green and cyan fluorescent protein chromophores investigated using action spectroscopy
264 1c 2023
338 a print2 rdacarrier
520 a The photophysics of biochromophore ions often depends on the isomeric or protomeric distribution, yet this distribution, and the individual isomer contributions to an action spectrum, can be difficult to quantify. Here, we use two separate photodissociation action spectroscopy instruments to record electronic spectra for protonated forms of the green (pHBDI(+)) and cyan (Cyan(+)) fluorescent protein chromophores. One instrument allows for cryogenic (T = 40 & PLUSMN; 10 K) cooling of the ions, while the other offers the ability to perform protomer-selective photodissociation spectroscopy. We show that both chromophores are generated as two protomers when using electrospray ionisation, and that the protomers have partially overlapping absorption profiles associated with the S-1 & LARR; S-0 transition. The action spectra for both species span the 340-460 nm range, although the spectral onset for the pHBDI(+) protomer with the proton residing on the carbonyl oxygen is red-shifted by & AP;40 nm relative to the lower-energy imine protomer. Similarly, the imine and carbonyl protomers are the lowest energy forms of Cyan(+), with the main band for the carbonyl protomer red-shifted by & AP;60 nm relative to the lower-energy imine protomer. The present strategy for investigating protomers can be applied to a wide range of other biochromophore ions.
650 7a NATURVETENSKAPx Kemi0 (SwePub)1042 hsv//swe
650 7a NATURAL SCIENCESx Chemical Sciences0 (SwePub)1042 hsv//eng
650 7a NATURVETENSKAPx Fysik0 (SwePub)1032 hsv//swe
650 7a NATURAL SCIENCESx Physical Sciences0 (SwePub)1032 hsv//eng
7001 a Dezalay, Jordanu Stockholms universitet,Fysikum4 aut0 (Swepub:su)jode5907
7001 a Ryan, Christopher R. M.4 aut
7001 a Ieritano, Christian4 aut
7001 a Hopkins, W. Scott4 aut
7001 a Chambrier, Isabelle4 aut
7001 a Cammidge, Andrew N.4 aut
7001 a Stockett, Mark H.,d 1984-u Stockholms universitet,Fysikum4 aut0 (Swepub:su)mstoc
7001 a Noble, Jennifer A.4 aut
7001 a Bull, James N.4 aut
7102 a Stockholms universitetb Fysikum4 org
7730 t Physical Chemistry, Chemical Physics - PCCPg 25:30, s. 20405-20413q 25:30<20405-20413x 1463-9076x 1463-9084
8564 u https://doi.org/10.1039/d3cp02661by Fulltext
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-221411
8564 8u https://doi.org/10.1039/d3cp02661b

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