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Gene regulation by low level UV-B radiation : identification by DNA array analysis

Brosché, Mikael (författare)
Schuler, Mary A. (författare)
Kalbina, Irina (författare)
Örebro universitet,Institutionen för naturvetenskap,Molekylär biokemi
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Connor, Lynn (författare)
Strid, Åke (författare)
Örebro universitet,Institutionen för naturvetenskap,Molekylär biokemi
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 (creator_code:org_t)
2002
2002
Engelska.
Ingår i: Photochemical and Photobiological Sciences. - 1474-905X .- 1474-9092. ; 1:9, s. 656-664
  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
Stäng  
  • UV-B radiation alters transcript levels of various defence genes and photosynthetic genes in plants. Utilising a DNA array with 5000 ESTs and cDNAs from Arabidopsis thaliana, 70 genes were found to show a greater than two-fold induction or repression of transcript levels. Six genes (MEB5.2, PyroA, Ubq3, Lhcb6, F5D21.10 and the gene for an RNA polymerase II subunit) were tested for stress specific gene regulation on northern blots with RNA from plants exposed to low dose UV-B radiation, ozone or wounding. Transcript levels for PyroA, Uhq3 and the gene for a RNA polymerase II subunit were all specifically increased by UV-B. MEB5.2 mRNA levels also rose, whereas Lhcb6 and FSD21.10 transcript levels decreased under all stresses. The PyroA gene product in fungi is needed for biosynthesis of pyridoxine, and might have a role in protection against singlet oxygen. The Ubq3 gene encodes the ubiquitin protein that is attached to proteins destined for degradation. MEB5.2 and F5D21.10 represent novel gene products whose function have not yet been identified. Pairwise comparisons between the UV-B inducible promoters have identified a series of elements present in the MEB5.2 and PyroA promoters, absent from promoters of genes for early phenylpropanoid metabolism and that may be responsible for modulating their UV-B responses.

Ämnesord

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

Nyckelord

Arabidopsis/genetics/radiation effects
Base Sequence
DNA; Plant/genetics
Expressed Sequence Tags
Gene Expression Regulation; Enzymologic/radiation effects
Gene Expression Regulation; Plant/*radiation effects
Molecular Sequence Data
Oligonucleotide Array Sequence Analysis
Plant Proteins/genetics
Promoter Regions (Genetics)
RNA Polymerase II/genetics
Ultraviolet Rays
Biochemistry
Biokemi
Biochemistry
Biokemi

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