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- Ncube, IgnatiousUniversity of Zimbabwe,Lund University (author)
Fatty acid selectivity of a lipase purified from Vernonia galamensis seed
- Article/chapterEnglish1995
Publisher, publication year, extent ...
- 1995
- 8 s.
Numbers
- LIBRIS-ID:oai:lup.lub.lu.se:6cc7a6cf-85c7-42a2-ba93-b77489be3776
- https://lup.lub.lu.se/record/6cc7a6cf-85c7-42a2-ba93-b77489be3776URI
- https://doi.org/10.1016/0005-2760(95)00067-MDOI
Supplementary language notes
- Language:English
- Summary in:English
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- SwePubSwePub
Classification
Notes
- Vernonia galamensis is an annual herb whose seed oil contains high levels of an epoxy fatty acid, vernolic (cis-12,13-epoxy cis-9-octadecenoic) acid. The seed also contains lipase activity in the dormant state. A lipase was purified from the seed and its substrate specificity studied in isooctane. The lipase shows pronounced selectivity for the native triacylglycerol, trivernolin. The rate of hydrolysis of triolein, the corresponding non epoxy triacylglycerol, is only 3% of that of trivernolin. In the acidolysis of tricaprylin using a mixture of fatty acids, the Vernonia lipase also showed selectivity for vernolic acid. Michaelis-Menten kinetics of the hydrolysis of triacylglycerols revealed that the observed high selectivity of the Vernonia lipase for trivernolin was mainly due to a higher Vmax for trivernolin. The Vmax value for the hydrolysis of trivernolin was 5 times higher than that for triolein. This novel substrate specificity is an adaptation by the seed lipase to the triacylglycerols of the seed oil that contain up to 80% vernolic acid.
Subject headings and genre
- TEKNIK OCH TEKNOLOGIER Industriell bioteknik Biokatalys och enzymteknik hsv//swe
- ENGINEERING AND TECHNOLOGY Industrial Biotechnology Biocatalysis and Enzyme Technology hsv//eng
- (V. galamensis)
- Fatty acid selectivity
- Lipase
- Purification
Added entries (persons, corporate bodies, meetings, titles ...)
- Gitlesen, ThomasLund University (author)
- Adlercreutz, PatrickLund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)biot-pad (author)
- Read, John S.University of Zimbabwe (author)
- Mattiasson, BoLund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)biot-bma (author)
- University of ZimbabweLund University (creator_code:org_t)
Related titles
- In:Biochimica et Biophysica Acta - Lipids and Lipid Metabolism1257:2, s. 149-1560005-2760
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