Profiling and quantifying endogenous molecules in single cells using nano-DESI MS

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Fältnamn	Indikatorer	Metadata
000		02848naa a2200325 4500
001		oai:DiVA.org:uu-336430
003		SwePub
008		171215s2017 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-3364302 URI
024	7	a https://doi.org/10.1039/c7an00885f2 DOI
040		a (SwePub)uu
041		a engb eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Bergman, Hilde-Marleneu Uppsala universitet,Analytisk kemi,Lanekoff4 aut0 (Swepub:uu)hilbe345
245	1 0	a Profiling and quantifying endogenous molecules in single cells using nano-DESI MS
264		c 2017
264	1	b ROYAL SOC CHEMISTRY,c 2017
338		a print2 rdacarrier
520		a Molecular profiling of single cells has the potential to significantly advance our understanding of cell function and cellular processes of importance to health and disease. In particular, small molecules with rapid turn-over rates can reveal activated metabolic pathways resulting from an altered chemical environment or cellular events such as differentiation. Consequently, techniques for quantitative metabolite detection acquired in a higher throughput manner are needed to characterize the biological variability between seemingly homogenous cells. Here, we show that nanospray desorption electrospray ionization (nano-DESI) mass spectrometry ( MS) enables sensitive molecular profiling and quantification of endogenous species in single cells in a higher throughput manner. Specifically, we show a large number of detected amino acids and phospholipids, including plasmalogens, readily detected from single cheek cells. Further, by incorporating a phosphatidylcholine ( PC) internal standard into the nano-DESI solvent, we determined the total amount of PC in one cell to be 1.2 pmoles. Finally, we describe a higher throughput approach where molecules in single cells are automatically profiled. These developments in single cell analysis provide a basis for future studies to understand cellular processes related to drug effects, cell differentiation and altered chemical microenvironments.
650	7	a NATURVETENSKAPx Kemix Analytisk kemi0 (SwePub)104012 hsv//swe
650	7	a NATURAL SCIENCESx Chemical Sciencesx Analytical Chemistry0 (SwePub)104012 hsv//eng
653		a Kemi med inriktning mot analytisk kemi
653		a Chemistry with specialization in Analytical Chemistry
700	1	a Lanekoff, Ingelau Uppsala universitet,Analytisk kemi,Lanekoff4 aut0 (Swepub:uu)ingla753
710	2	a Uppsala universitetb Analytisk kemi4 org
773	0	t The Analystd : ROYAL SOC CHEMISTRYg 142:19, s. 3639-3647q 142:19<3639-3647x 0003-2654x 1364-5528
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-336430
856	4 8	u https://doi.org/10.1039/c7an00885f

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Av författaren/redakt...: Bergman, Hilde-M ...; Lanekoff, Ingela

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