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N-terminal peptides...
N-terminal peptides from unprocessed prion proteins enter cells by macropinocytosis
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- Magzoub, Mazin (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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- Sandgren, Staffan (author)
- Lund University,Lunds universitet,Bröstcancer-genetik,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Breastcancer-genetics,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine
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- Pontus, Lundberg (author)
- Stockholms universitet,Institutionen för neurokemi
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- Oglecka, Kamila (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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Lilja, Johanna (author)
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- Wittrup, Anders (author)
- Lund University,Lunds universitet,Bröstcancer-genetik,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Breastcancer-genetics,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine
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- Eriksson, L. E. Göran (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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- Langel, Ülo (author)
- Stockholms universitet,Institutionen för neurokemi
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- Belting, Mattias (author)
- Lund University,Lunds universitet,Bröstcancer-genetik,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Breastcancer-genetics,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine
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- Astrid, Gräslund (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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- Welch, Johanna (author)
- Lund University,Lunds universitet,Bröstcancer-genetik,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Breastcancer-genetics,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine
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(creator_code:org_t)
- Elsevier BV, 2006
- 2006
- English.
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In: Biochemical and Biophysical Research Communications - BBRC. - : Elsevier BV. - 0006-291X .- 1090-2104. ; 348:2, s. 379-385
- Related links:
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http://dx.doi.org/10...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
Close
- A peptide derived from the N-terminus of the unprocessed bovine prion protein (bPrPp), incorporating the hydrophobic signal sequence (residues 1–24) and a basic domain (KKRPKP, residues 25–30), internalizes into mammalian cells, even when coupled to a sizeable cargo, and therefore functions as a cell-penetrating peptide (CPP). Confocal microscopy and co-localization studies indicate that the internalization of bPrPp is mainly through macropinocytosis, a fluid-phase endocytosis process, initiated by binding to cell-surface proteoglycans. Electron microscopy studies show internalized bPrPp–DNA–gold complexes residing in endosomal vesicles. bPrPp induces expression of a complexed luciferase-encoding DNA plasmid, demonstrating the peptide’s ability to transport the cargo across the endosomal membrane and into the cytosol and nucleus. The novel CPP activity of the unprocessed N-terminal domain of PrP could be important for the retrotranslocation of partly processed PrP and for PrP trafficking inside or between cells, with implications for the infectivity associated with prion diseases.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Keyword
- Prion protein
- N-terminus
- Cell-penetrating peptide
- Endocytosis
- Macropinocytosis
- Proteoglycan
- proteoglycan
- macropinocytosis
- endocytosis
- cell-penetrating peptide
- prion protein
- N-terminus
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Magzoub, Mazin
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Sandgren, Staffa ...
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Pontus, Lundberg
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Oglecka, Kamila
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Lilja, Johanna
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Wittrup, Anders
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Eriksson, L. E. ...
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Langel, Ülo
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Belting, Mattias
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Astrid, Gräslund
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Welch, Johanna
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
- Articles in the publication
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Biochemical and ...
- By the university
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Stockholm University
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Lund University