Platelet membranes induce airway smooth muscle cell proliferation

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Fältnamn	Indikatorer	Metadata
000		04326naa a2200493 4500
001		oai:DiVA.org:oru-17160
003		SwePub
008		110902s2011 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
009		oai:DiVA.org:liu-61615
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-171602 URI
024	7	a https://doi.org/10.3109/09537104.2010.5156962 DOI
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-616152 URI
040		a (SwePub)orud (SwePub)liu
041		a engb eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Svensson Holm, Ann-Charlotte B.u Linköpings universitet,Farmakologi,Hälsouniversitetet4 aut0 (Swepub:liu)annsv86
245	1 0	a Platelet membranes induce airway smooth muscle cell proliferation
264		c 2011-01-03
264	1	b Informa Healthcare,c 2011
338		a print2 rdacarrier
500		a Original Publication: Ann-Charlotte B. Svensson Holm, Torbjörn Bengtsson, Magnus Grenegård and Eva G. Lindström, Platelet membranes induce airway smooth muscle cellproliferation, 2011, Platelets, (22), 1, 45-55. http://dx.doi.org/10.3109/09537104.2010.515696 Copyright: Informa Healthcare http://informahealthcare.com/
520		a The role of platelets in airway disease is poorly understood although they have been suggested to influence on proliferation of airway smooth muscle cells (ASMC). Platelets have been found localized in the airways in autopsy material from asthmatic patients and have been implicated in airway remodeling. The aim of the present study was to investigate the effects of various platelet fractions on proliferation of ASMC obtained from guinea pigs (GP-ASMC) and humans (H-ASMC). Proliferation of ASMC was measured by the MTS assay and the results confirmed by measurements of the DNA content. A key observation was that the platelet membrane preparations induced a significant increase in the proliferation of both GP-ASMC (129.9 +/- 3.0 %) and H-ASMC (144.8 +/- 12.2). However, neither supernatants from lysed or filtrated thrombin stimulated platelets induced ASMC proliferation to the same extent as the membrane preparation. We have previously shown that platelet-induced proliferation is dependent on 5-lipoxygenase (5-LOX) and reactive oxygen species (ROS) pathways. In the present work we established that platelet membrane-induced ASMC proliferation was reduced in the presence of the NADPH oxidase inhibitor DPI and the 5-LOX inhibitor AA-861. In conclusion, our results showed that platelet membranes significantly induced ASMC proliferation, demonstrating that the mitogenic effect of platelets and platelet membranes on ASMC is mainly due to membrane-associated factors. The effects of platelet membranes were evident on both GP-ASMC and H-ASMC and involved 5-LOX and ROS. These new findings are of importance in understanding the mechanisms contributing to airway remodeling and may contribute to the development of new pharmacological tools in the treatment of inflammatory airway diseases.
653		a Platelets
653		a platelet membranes
653		a airway smooth muscle cell
653		a 5-lipoxygenase
653		a reactive oxygen species
653		a airway remodeling
653		a MEDICINE
653		a MEDICIN
653		a Medicin
653		a Medicine
700	1	a Bengtsson, Torbjörnu Örebro universitet,Hälsoakademin,Department of Biomedicine, School of Health and Medical Sciences, Örebro University, Sweden4 aut0 (Swepub:oru)tbn
700	1	a Grenegård, Magnusu Linköpings universitet,Farmakologi,Hälsouniversitetet4 aut0 (Swepub:liu)maggr06
700	1	a Lindström, Eva G.u Linköpings universitet,Farmakologi,Hälsouniversitetet4 aut0 (Swepub:liu)evali73
710	2	a Linköpings universitetb Farmakologi4 org
773	0	t Plateletsd : Informa Healthcareg 22:1, s. 45-55q 22:1<45-55x 0953-7104x 1369-1635
856	4	u http://liu.diva-portal.org/smash/get/diva2:370611/FULLTEXT01
856	4	u https://liu.diva-portal.org/smash/get/diva2:370611/FULLTEXT01.pdfx primaryx Raw objecty fulltext:postprint
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-17160
856	4 8	u https://doi.org/10.3109/09537104.2010.515696
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-61615

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