The minimum information required for a glycomics experiment (MIRAGE) project: improving the standards for reporting glycan microarray-based data

• MIRAGE (Minimum Information Required for A Glycomics Experiment) is an initiative that was created by experts in the fields of glycobiology, glycoanalytics and glycoinformatics to produce guidelines for reporting results from the diverse types of experiments and analyses used in structural and functional studies of glycans in the scientific literature. As a sequel to the guidelines for sample preparation (Struwe et al. 2016, Glycobiology, 26: 907-910) and mass spectrometry data (Kolarich et al. 2013, Mol. Cell Proteomics, 12: 991-995), here we present the first version of guidelines intended to improve the standards for reporting data from glycan microarray analyses. For each of eight areas in the workflow of a glycan microarray experiment, we provide guidelines for the minimal information that should be provided in reporting results. We hope that the MIRAGE glycan microarray guidelines proposed here will gain broad acceptance by the community, and will facilitate interpretation and reproducibility of the glycan microarray results with implications in comparison of data from different laboratories and eventual deposition of glycan microarray data in international databases.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)

Nyckelord

glycan microarrays

glycans

glycobiology

glycomics

MIRAGE

shotgun glycomics

mass-spectrometry

binding-proteins

experiment

miame

array data

receptors

oligosaccharides

neoglycolipids

specificities

biomarkers

B 440

S-40530 Gothenburg

Sweden.

[Khoo

Kay-Hooi] Acad Sinica

Inst Biol Chem

128

Acad Rd Sec 2

Taipei 115

Taiwan.

[Kolarich

Daniel] Max Planck Inst Colloids & Interfaces

Dept Biomol Syst

D-14424 Potsdam

Germany.

[Novotny

Milos V.] Indiana Univ

Dept Chem

800 E Kirkwood Ave

Bloomington

IN 47405 USA.

[Ranzinger

Rene

Tiemeyer

Michael

Wells

Lance

York

William S.] Univ Georgia

Complex

[Rapp

Erdmann] Max Planck Inst Dynam Complex Tech Syst

Bioproc Engn

D-39106 Magdeburg

[Rudd

Pauline M.] NIBRT Natl Inst Bioproc Res & Training

NIBRT GlycoSci Grp

Fosters Ave

Blackrock

[Struwe

Weston B.] Univ Oxford

Glycobiol Inst

Dept Biochem

Oxford OX1 3QU

England.

[Kettner

Carsten] Beilstein Inst

Trakehner Str 7-9

D-60487 Frankfurt

Germany.

[Smith

David F.] Emory Univ

Sch Med

Emory Comprehens Glyc Core

Atlanta

GA 30322 USA.

ng pw

1985

biochemical and biophysical research communications

v132

p474

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