Search: WFRF:(Sandberg Mats 1953)
> (2010-2014) >
Assessment of tissu...
Assessment of tissue viability following electroosmotic push-pull perfusion from organotypic hippocampal slice cultures
-
Rupert, A. E. (author)
-
Ou, Y. (author)
-
- Sandberg, Mats, 1953 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
-
show more...
-
Weber, S. G. (author)
-
show less...
-
(creator_code:org_t)
- 2013-05-02
- 2013
- English.
-
In: ACS Chemical Neuroscience. - : American Chemical Society (ACS). - 1948-7193. ; 4:5, s. 849-857
- Related links:
-
https://europepmc.or...
-
show more...
-
https://gup.ub.gu.se...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- We have developed a novel sampling technique that allows both introduction and removal of fluid from the extracellular space of living tissue. This method is based on the fluidics of push-pull perfusion but flow is driven by electroosmosis. We have applied this method to organotypic hippocampal cultures. A source capillary is inserted into the tissue and a collection capillary is in contact with the tissue surface through a thin layer of fluid. A voltage is applied across the proximal ends of source and collection capillary. In the applied field, fluid will move from source, into the tissue, and then be collected. In this process, damage to cells may occur. To understand better what sampling conditions influence damage most, we tested various sampling geometries and applied voltages, quantifying damage 16-24 h later using propidium iodide as a cell death marker. We found that damage correlates with both voltage drop and power dissipated in the tissue, but that voltage drop is a better indicator of damage when comparing models in which capillary arrangement and length are different. © 2013 American Chemical Society.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Keyword
- electroosmosis
- injury
- Organotypic hippocampal cultures
- push-pull perfusion
- sampling
- propidium iodide
- animal experiment
- article
- brain perfusion
- capillary
- cell death
- controlled study
- electric field
- electric potential
- extracellular space
- geometry
- hippocampus
- nonhuman
- priority journal
- rat
- Animals
- Electric Stimulation
- Organ Culture Techniques
- Perfusion
- Rats
- Rats
- Sprague-Dawley
- Tissue Survival
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database