Quantitative mass spectrometry imaging of small-molecule neurotransmitters in rat brain tissue sections using nanospray desorption electrospray ionization

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Fältnamn	Indikatorer	Metadata
000		02809naa a2200325 4500
001		oai:DiVA.org:uu-281314
003		SwePub
008		160322s2016 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-2813142 URI
024	7	a https://doi.org/10.1039/c5an02620b2 DOI
040		a (SwePub)uu
041		a engb eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Bergman, Hilde-Marléneu Uppsala universitet,Analytisk kemi,Lanekoff group4 aut0 (Swepub:uu)hilbe345
245	1 0	a Quantitative mass spectrometry imaging of small-molecule neurotransmitters in rat brain tissue sections using nanospray desorption electrospray ionization
264		c 2016
264	1	b Royal Society of Chemistry (RSC),c 2016
338		a print2 rdacarrier
520		a Small molecule neurotransmitters are essential for the function of the nervous system, and neurotransmitter imbalances are often connected to neurological disorders. The ability to quantify such imbalances is important to provide insights into the biochemical mechanisms underlying the disorder. This proof-of-principle study presents online quantification of small molecule neurotransmitters, specifically acetylcholine, γ-aminobutyric acid (GABA) and glutamate, in rat brain tissue sections using nanospray desorption electrospray ionization (nano-DESI) mass spectrometry imaging. By incorporating deuterated internal standards in the nano-DESI solvent we show identification, accurate mapping, and quantification of these small neurotransmitters in rat brain tissue without introducing any additional sample preparation steps. We find that GABA is about twice as abundant in the medial septum-diagonal band complex (MSDB) as in the cortex, while glutamate is about twice as abundant in the cortex as compared to the MSDB. The study shows that nano-DESI is well suited for imaging of small molecule neurotransmitters in health and disease.
650	7	a NATURVETENSKAPx Kemix Analytisk kemi0 (SwePub)104012 hsv//swe
650	7	a NATURAL SCIENCESx Chemical Sciencesx Analytical Chemistry0 (SwePub)104012 hsv//eng
700	1	a Lundin, Eriku Uppsala universitet,Analytisk kemi,Lanekoff group4 aut
700	1	a Andersson, Malinu Uppsala universitet,Institutionen för farmaceutisk biovetenskap4 aut0 (Swepub:uu)malan799
700	1	a Lanekoff, Ingela,c Assoc. Prof.d 1975-u Uppsala universitet,Analytisk kemi,Lanekoff group4 aut0 (Swepub:uu)ingla753
710	2	a Uppsala universitetb Analytisk kemi4 org
773	0	t The Analystd : Royal Society of Chemistry (RSC)g 141:12, s. 3686-3695q 141:12<3686-3695x 0003-2654x 1364-5528
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-281314
856	4 8	u https://doi.org/10.1039/c5an02620b

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Av författaren/redakt...: Bergman, Hilde-M ...; Lundin, Erik; Andersson, Malin; Lanekoff, Ingela ...

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