Sökning: WFRF:(Andersson Maria 1969 ) > Evaluation of refer...
Fältnamn | Indikatorer | Metadata |
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000 | 05106naa a2200493 4500 | |
001 | oai:gup.ub.gu.se/227559 | |
003 | SwePub | |
008 | 240910s2015 | |||||||||||000 ||eng| | |
009 | oai:DiVA.org:uu-278138 | |
024 | 7 | a https://gup.ub.gu.se/publication/2275592 URI |
024 | 7 | a https://doi.org/10.1080/21623945.2015.10398842 DOI |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-2781382 URI |
040 | a (SwePub)gud (SwePub)uu | |
041 | a eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Taube, Magdalenau Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för molekylär och klinisk medicin,Institute of Medicine, Department of Molecular and Clinical Medicine4 aut0 (Swepub:gu)xtaubm |
245 | 1 0 | a Evaluation of reference genes for gene expression studies in human brown adipose tissue. |
264 | c 2015-05-28 | |
264 | 1 | b Informa UK Limited,c 2015 |
520 | a Human brown adipose tissue (BAT) has during the last 5 year been subjected to an increasing research interest, due to its putative function as a target for future obesity treatments. The most commonly used method for molecular studies of human BAT is the quantitative polymerase chain reaction (qPCR). This method requires normalization to a reference gene (genes with uniform expression under different experimental conditions, e.g. similar expression levels between human BAT and WAT), but so far no evaluation of reference genes for human BAT has been performed. Two different microarray datasets with samples containing human BAT were used to search for genes with low variability in expression levels. Seven genes (FAM96B, GNB1, GNB2, HUWE1, PSMB2, RING1 and TPT1) identified by microarray analysis, and 8 commonly used reference genes (18S, B2M, GAPDH, LRP10, PPIA, RPLP0, UBC, and YWHAZ) were selected and further analyzed by quantitative PCR in both BAT containing perirenal adipose tissue and subcutaneous adipose tissue. Results were analyzed using 2 different algorithms (Normfinder and geNorm). Most of the commonly used reference genes displayed acceptably low variability (geNorm M-values <0.5) in the samples analyzed, but the novel reference genes identified by microarray displayed an even lower variability (M-values <0.25). Our data suggests that PSMB2, GNB2 and GNB1 are suitable novel reference genes for qPCR analysis of human BAT and we recommend that they are included in future gene expression studies of human BAT. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Kirurgi0 (SwePub)302122 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Surgery0 (SwePub)302122 hsv//eng |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Cell- och molekylärbiologi0 (SwePub)301082 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Cell and Molecular Biology0 (SwePub)301082 hsv//eng |
653 | a brown adipose tissue | |
653 | a human | |
653 | a quantitative PCR | |
653 | a reference genes | |
653 | a white adipose tissue | |
700 | 1 | a Andersson-Assarsson, Johanna C.,d 1974u Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för molekylär och klinisk medicin,Institute of Medicine, Department of Molecular and Clinical Medicine4 aut0 (Swepub:gu)xanjoh |
700 | 1 | a Lindberg, Kristinu Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för molekylär och klinisk medicin,Institute of Medicine, Department of Molecular and Clinical Medicine4 aut |
700 | 1 | a Pereira, Maria J,d 1981u Uppsala universitet,Klinisk diabetologi och metabolism4 aut0 (Swepub:uu)marpe927 |
700 | 1 | a Gäbel, Markusu Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för kirurgi,Institute of Clinical Sciences, Department of Surgery4 aut0 (Swepub:gu)xgabem |
700 | 1 | a Svensson, Maria Ku Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för molekylär och klinisk medicin,Institute of Medicine, Department of Molecular and Clinical Medicine4 aut0 (Swepub:gu)xsmara |
700 | 1 | a Eriksson, Jan W,d 1959u Uppsala universitet,Klinisk diabetologi och metabolism4 aut0 (Swepub:uu)janer909 |
700 | 1 | a Svensson, Per-Arne,d 1969u Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för molekylär och klinisk medicin,Institute of Medicine, Department of Molecular and Clinical Medicine4 aut0 (Swepub:gu)xsvper |
710 | 2 | a Göteborgs universitetb Institutionen för medicin, avdelningen för molekylär och klinisk medicin4 org |
773 | 0 | t Adipocyted : Informa UK Limitedg 4:4, s. 280-5q 4:4<280-5x 2162-3945x 2162-397X |
856 | 4 | u https://www.tandfonline.com/doi/pdf/10.1080/21623945.2015.1039884?needAccess=true |
856 | 4 8 | u https://gup.ub.gu.se/publication/227559 |
856 | 4 8 | u https://doi.org/10.1080/21623945.2015.1039884 |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-278138 |
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