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Search: WFRF:(Engqvist R) > HAG2/MYB76 and HAG3...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00003282naa a2200421 4500
001oai:research.chalmers.se:3d175bb9-c491-4d0f-bf80-b43b12b1ebf1
003SwePub
008171007s2008 | |||||||||||000 ||eng|
024a https://research.chalmers.se/publication/2481032 URI
024a https://doi.org/10.1111/j.1469-8137.2007.02295.x2 DOI
040 a (SwePub)cth
041 a engb eng
042 9 SwePub
072 7a art2 swepub-publicationtype
072 7a ref2 swepub-contenttype
100a Gigolashvili, T.4 aut
2451 0a HAG2/MYB76 and HAG3/MYB29 exert a specific and coordinated control on the regulation of aliphatic glucosinolate biosynthesis in Arabidopsis thaliana
264 c 2007-11-28
264 1b Wiley,c 2008
520 a In a previous transactivation screen, two Arabidopsis thaliana R2R3-MYB transcription factors, HAG2/MYB76 and HAG3/MYB29, along with the already characterized HAG1/MYB28, were identified as putative regulators of aliphatic glucosinolate biosynthesis. • Molecular and biochemical characterization of HAG2/MYB76 and HAG3/MYB29 functions was performed using transformants with increased or repressed transcript levels. Real-time PCR assays, cotransformation assays and measurements of glucosinolate contents were used to assess the impact of both MYB factors on the steady-state level of glucosinolate biosynthetic genes and accumulation of aliphatic glucosinolates. • Both HAG2/MYB76 and HAG3/MYB29 were shown to be positive regulators of aliphatic glucosinolate biosynthesis. Expression of promoter-β- glucuronidase (GUS) fusions indicated GUS activities in both vegetative and generative organs, with distinct characteristics for each MYB factor. HAG1/MYB28, HAG2/MYB76 and HAG3/MYB29 reciprocally transactivated each other in the control of aliphatic glucosinolate biosynthesis and downregulated the expression of genes involved in the control of indolic glucosinolate biosynthesis, pointing to a reciprocal negative regulation of these two pathways. • All three HAG transcription factors exert a coordinated control on aliphatic glucosinolate biosynthesis.
650 7a NATURVETENSKAPx Biologix Botanik0 (SwePub)106072 hsv//swe
650 7a NATURAL SCIENCESx Biological Sciencesx Botany0 (SwePub)106072 hsv//eng
650 7a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe
650 7a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng
653 a Methyl jasmonate-responsive genes
653 a Glucosinolates
653 a MYB transcription factors
653 a Regulatory network
653 a Transactivation
700a Engqvist, Martin,d 1983u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)marengq
700a Yatusevich, R.4 aut
700a Müller, C.4 aut
700a Flügge, U.I.4 aut
710a Chalmers tekniska högskola4 org
773t New Phytologistd : Wileyg 177:3, s. 627-642q 177:3<627-642x 1469-8137x 0028-646X
856u https://nph.onlinelibrary.wiley.com/doi/pdfdirect/10.1111/j.1469-8137.2007.02295.x
8564 8u https://research.chalmers.se/publication/248103
8564 8u https://doi.org/10.1111/j.1469-8137.2007.02295.x

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