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Higher cardiogenic ...
Higher cardiogenic potential of iPSCs derived from cardiac versus skin stromal cells
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Meraviglia, Viviana (author)
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Wen, Jianyan (author)
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Piacentini, Luca (author)
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Campostrini, Giulia (author)
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Wang, Cheng (author)
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Florio, Maria Cristina (author)
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Azzimato, Valerio (author)
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Fassina, Lorenzo (author)
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Langes, Martin (author)
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Wong, Johnson (author)
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Miragoli, Michele (author)
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Gaetano, Carlo (author)
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Pompilio, Giulio (author)
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Barbuti, Andrea (author)
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DiFrancesco, Dario (author)
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- Mascalzoni, Deborah (author)
- Center for Biomedicine, European Academy Bozen/Bolzano (EURAC) (affiliated institute of the University of Lübeck), Bolzano, 39100 Italy.
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Pramstaller, Peter P (author)
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Colombo, Gualtiero I (author)
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Chen, Huei-Sheng Vincent (author)
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Rossini, Alessandra (author)
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Center for Biomedicine, European Academy Bozen/Bolzano (EURAC) (affiliated institute of the University of Lübeck), Bolzano, 39100 Italy (creator_code:org_t)
- 2016
- 2016
- English.
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In: Frontiers in Bioscience. - 1093-9946 .- 1093-4715. ; 21, s. 719-43
- Related links:
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https://urn.kb.se/re...
Abstract
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- Prior studies have demonstrated that founder cell type could influence induced pluripotent stem cells (iPSCs) molecular and developmental properties at early passages after establishing their pluripotent state. Herein, we evaluated the persistence of a functional memory related to the tissue of origin in iPSCs from syngeneic cardiac (CStC) vs skin stromal cells (SStCs). We found that, at passages greater than 15, iPSCs from cardiac stromal cells (C-iPSCs) produced a higher number of beating embryoid bodies than iPSCs from skin stromal cells (S-iPSCs). Flow cytometry analysis revealed that dissected beating areas from C-iPSCs exhibited more Troponin-T positive cells compared to S-iPSCs. Beating areas derived from C-iPSCs displayed higher expression of cardiac markers, more hyperpolarized diastolic potentials, larger action potential amplitude and higher contractility than beaters from skin. Also, different microRNA subsets were differentially modulated in CStCs vs SStCs during the reprogramming process, potentially accounting for the higher cardiogenic potentials of C-iPSCs vs S-iPSCs. Therefore, the present work supports the existence of a founder organ memory in iPSCs obtained from the stromal component of the origin tissue.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
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- art (subject category)
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- By the author/editor
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Meraviglia, Vivi ...
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Wen, Jianyan
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Piacentini, Luca
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Campostrini, Giu ...
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Wang, Cheng
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Florio, Maria Cr ...
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show more...
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Azzimato, Valeri ...
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Fassina, Lorenzo
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Langes, Martin
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Wong, Johnson
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Miragoli, Michel ...
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Gaetano, Carlo
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Pompilio, Giulio
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Barbuti, Andrea
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DiFrancesco, Dar ...
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Mascalzoni, Debo ...
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Pramstaller, Pet ...
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Colombo, Gualtie ...
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Chen, Huei-Sheng ...
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Rossini, Alessan ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
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Frontiers in Bio ...
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Uppsala University