Transforming growth factor-beta1-regulated proteins in human endothelial cells identified by two-dimensional gel electrophoresis and mass spectrometry

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Lomnytska, MartaUppsala universitet,Ludwiginstitutet för cancerforskning (författare)

Transforming growth factor-beta1-regulated proteins in human endothelial cells identified by two-dimensional gel electrophoresis and mass spectrometry

Artikel/kapitelEngelska2004

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Wiley,2004
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LIBRIS-ID:oai:DiVA.org:uu-72987
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-72987URI
https://doi.org/10.1002/pmic.200300659DOI

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Språk:engelska
Sammanfattning på:engelska &language:-1_t

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Ämneskategori:art swepub-publicationtype

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Transforming growth factor-beta (TGFbeta) is a potent regulator of angiogenesis affecting proliferation, differentiation and migration of endothelial cells. The effect of TGFbeta on endothelial cells depends on the origin of the cells and on the experimental conditions. Global analysis of TGFbeta signalling is expected to unveil mechanisms of this variability and identify novel targets of the growth factor. Here, we report proteome profiling of human microvascular endothelial cells obtained from dermis, which were treated with TGFbeta1 and compared to nontreated cells. We identified 54 proteins affected by TGFbeta1 using two-dimensional gel electrophoresis and peptide mass fingerprinting. Thirteen of the identified proteins are involved in various signalling processes. Seven proteins are involved in cytoskeleton rearrangements and six are involved in regulation of metabolism. Ten proteins were identical to predicted hypothetical proteins with no assigned functions. In agreement with the effect of TGFbeta1 on components of the cytoskeleton, TGFbeta1 induces actin cytoskeleton rearrangements. TGFbeta1 also affected expression of E2F6, p57Kip2, G(q)alpha, hnRNP A1 and myosin light chain proteins as shown by immunoblotting. Down-regulation of the transcriptional repressor E2F6 by TGFbeta1 correlated with a weak growth-inhibitory activity of TGFbeta1 on HMVEC-d cells. Twenty-five of the identified proteins have not previously been described as being regulated by TGFbeta1, providing new insights into TGFbeta1 signalling in endothelial cells.

Ämnesord och genrebeteckningar

Cells; Cultured
Cytoskeleton/*metabolism
Dermis
Electrophoresis; Gel; Two-Dimensional
Endothelial Cells/*metabolism
Humans
Peptide Mapping
Proteome
Research Support; Non-U.S. Gov't
Signal Transduction/*physiology
Transforming Growth Factor beta/*metabolism

Biuppslag (personer, institutioner, konferenser, titlar ...)

Lukiyanchuk, VasylUppsala universitet,Ludwiginstitutet för cancerforskning(Swepub:uu)valuk169 (författare)
Hellman, UlfUppsala universitet,Ludwiginstitutet för cancerforskning(Swepub:uu)ulfhm (författare)
Souchelnytskyi, SerhiyUppsala universitet,Ludwiginstitutet för cancerforskning(Swepub:uu)sergsouc (författare)
Uppsala universitetLudwiginstitutet för cancerforskning (creator_code:org_t)

Sammanhörande titlar

Ingår i:Proteomics: Wiley4:4, s. 995-10061615-98531615-9861

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Av författaren/redakt...: Lomnytska, Marta; Lukiyanchuk, Vas ...; Hellman, Ulf; Souchelnytskyi, ...

Artiklar i publikationen: Proteomics

Av lärosätet: Uppsala universitet

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