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Sökning: WFRF:(Wang Jian) > (2015-2019) > Genome sequence of ...

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FältnamnIndikatorerMetadata
00004051naa a2200649 4500
001oai:DiVA.org:umu-158120
003SwePub
008190412s2019 | |||||||||||000 ||eng|
024a https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-1581202 URI
024a https://doi.org/10.1093/gigascience/giy1642 DOI
040 a (SwePub)umu
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
100a Xu, Chao-Qun4 aut
2451 0a Genome sequence of Malania oleifera, a tree with great value for nervonic acid production
264 c 2019-01-24
264 1b Oxford University Press,c 2019
338 a electronic2 rdacarrier
520 a Background Malania oleifera, a member of the Olacaceae family, is an IUCN red listed tree, endemic and restricted to the Karst region of southwest China. This tree's seed is valued for its high content of precious fatty acids (especially nervonic acid). However, studies on its genetic makeup and fatty acid biogenesis are severely hampered by a lack of molecular and genetic tools. Findings We generated 51 Gb and 135Gb of raw DNA sequences, using Pacific Biosciences (PacBio) single-molecule real-time and 10x Genomics sequencing, respectively. A final genome assembly, with a scaffold N50 size of 4.65 Mb and a total length of 1.51Gb, was obtained by primary assembly based on PacBio long reads plus scaffolding with 10x Genomics reads. Identified repeats constituted approximate to 82% of the genome, and 24,064 protein-coding genes were predicted with high support. The genome has low heterozygosity and shows no evidence for recent whole genome duplication. Metabolic pathway genes relating to the accumulation of long-chain fatty acid were identified and studied in detail. Conclusions Here, we provide the first genome assembly and gene annotation for M. oleifera. The availability of these resources will be of great importance for conservation biology and for the functional genomics of nervonic acid biosynthesis.
650 7a NATURVETENSKAPx Biologix Genetik0 (SwePub)106092 hsv//swe
650 7a NATURAL SCIENCESx Biological Sciencesx Genetics0 (SwePub)106092 hsv//eng
653 a de novo genome assembly
653 a vulnerable plant
653 a Malania
653 a nervonic acid
653 a transcriptomes
700a Liu, Hui4 aut
700a Zhou, Shan-Shan4 aut
700a Zhang, Dong-Xu4 aut
700a Zhao, Wei4 aut
700a Wang, Sihai4 aut
700a Chen, Fu4 aut
700a Sun, Yan-Qiang4 aut
700a Nie, Shuai4 aut
700a Jia, Kai-Hua4 aut
700a Jiao, Si-Qian4 aut
700a Zhang, Ren-Gang4 aut
700a Yun, Quan-Zheng4 aut
700a Guan, Wenbin4 aut
700a Wang, Xuewen4 aut
700a Gao, Qiong4 aut
700a Bennetzen, Jeffrey L.4 aut
700a Maghuly, Fatemeh4 aut
700a Porth, Ilga4 aut
700a Van de Peer, Yves4 aut
700a Wang, Xiao-Ruu Umeå universitet,Institutionen för ekologi, miljö och geovetenskap,Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, School of Nature Conservation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China4 aut0 (Swepub:umu)xiwa0003
700a Ma, Yongpeng4 aut
700a Mao, Jian-Feng4 aut
710a Umeå universitetb Institutionen för ekologi, miljö och geovetenskap4 org
773t GigaScienced : Oxford University Pressg 8:2q 8:2x 2047-217X
856u https://doi.org/10.1093/gigascience/giy164y Fulltext
856u https://umu.diva-portal.org/smash/get/diva2:1304461/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print
856u https://academic.oup.com/gigascience/article-pdf/8/2/giy164/28238723/giy164.pdf
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-158120
8564 8u https://doi.org/10.1093/gigascience/giy164

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