(L4X0:1651 6214) srt2:(2020-2024) mspu:(artistic)
Search: (L4X0:1651 6214) srt2:(2020-2024) mspu:(artistic) > Engineering Surface...
- 2 of 2
- Previous record
- Next record
- To hitlist
Engineering Surfaces of Solid-State Nanopores for Biomolecule Sensing
-
- Li, Shiyu, 1991- (author)
- Uppsala universitet,Fasta tillståndets elektronik
-
- Zhang, Shi-Li, Professor (thesis advisor)
- Uppsala universitet,Fasta tillståndets elektronik
-
- Mayer, Michael, Professor (opponent)
- Adolphe Merkle Institute, University of Fribourg
- (creator_code:org_t)
- ISBN 9789151311296
- Uppsala : Acta Universitatis Upsaliensis, 2021
- English 91 s.
- Series: Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, 1651-6214 ; 2010
- Artistic work (other academic/artistic)
Abstract
Subject headings
Close
- Nanopores have emerged as a special class of single-molecule analytical tool that offers immense potential for sensing and characterizing biomolecules such as nucleic acids and proteins. As an alternative to biological nanopores, solid-state nanopores present remarkable versatility due to their wide-range tunability in pore geometry and dimension as well as their excellent mechanical robustness and stability. However, being intrinsically incompatible with biomolecules, surfaces of inorganic solids need be modified to provide desired functionalities for real-life sensing purposes. In this thesis, we presented an exploration of various surface engineering strategies and an examination of several surface associated phenomena pertaining specifically to solid-state nanopores. Based on the parallel sensing concept using arrayed pores, optical readout is mainly employed throughout the whole study.For the surface engineering aspect, a list of approaches was explored. A versatile surface patterning strategy for immobilization of biomolecules was developed based on selective poly(vinylphosphonic acid) passivation and electron beam induced deposition technique. This scheme was then implemented on nanopore arrays for nanoparticle localization. In addition, vesicle rupture-based lipid bilayer coating was adapted to truncated-pyramidal nanopores, which was shown to be effective for the minimizing DNA-pore interaction. Further, HfO2 coating by means of atomic layer deposition was employed to prevent the erosion of Si-based pores and to shrink the pore diameter, which enabled reliable investigations of DNA clogging and DNA polymerase docking.For the surface associated phenomena, several findings were made. The lipid bilayer formation on truncated pyramidal nanopores via instantaneous rupture of individual vesicles was quantified based on combined ionic current monitoring and optical observation. The probability of pore clogging appeared to linearly increase with the length of DNA strands and applied bias voltage, which could be attributed a higher probability of knotting and/or folding of longer DNA strands and more frequent translocation events at higher voltage. A free-energy based analytical model was proposed to evaluate the DNA-pore interaction and to interpret observed clogging behavior. Finally, docking of DNA polymerase on nanopore arrays was demonstrated using label-free optical method based on Ca2+ indicator dyes, which may open the avenue to sequencing-by-synthesis enabled by the docked polymerase.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Elektroteknik och elektronik -- Annan elektroteknik och elektronik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Electrical Engineering, Electronic Engineering, Information Engineering -- Other Electrical Engineering, Electronic Engineering, Information Engineering (hsv//eng)
Keyword
- solid-state nanopores
- surface engineering
- DNA sensing
- DNA polymerase
- optical detection
- lipid bilayer
- hafnium oxide
- electron beam induced deposition
Publication and Content Type
- vet (subject category)
- kfu (subject category)
- dok (subject category)
Find in a library
- Engineering Surfaces of Solid-State Nanopores for Biomolecule Sensing (Search the publication in LIBRIS)
To the university's database
- 2 of 2
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Li, Shiyu, 1991-
- Zhang, Shi-Li, P ...
- Mayer, Michael, ...
- About the subject
-
- ENGINEERING AND TECHNOLOGY
- ENGINEERING AND ...
- and Electrical Engin ...
- and Other Electrical ...
- Parts in the series
- Digital Comprehe ...
- By the university
- Uppsala University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
