(WFRF:(Krunic M)) srt2:(2018) spr:eng
Sökning: (WFRF:(Krunic M)) srt2:(2018) spr:eng > Clathrin coat contr...
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 03711naa a2200421 4500 | |
| 001 | oai:DiVA.org:kth-227777 | |
| 003 | SwePub | |
| 008 | 180514s2018 | |||||||||||000 ||eng| | |
| 009 | oai:prod.swepub.kib.ki.se:138152948 | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-2277772 URI |
| 024 | 7 | a https://doi.org/10.7554/eLife.325692 DOI |
| 024 | 7 | a http://kipublications.ki.se/Default.aspx?queryparsed=id:1381529482 URI |
| 040 | a (SwePub)kthd (SwePub)ki | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Farsi, Zohrehu Max Planck Inst Biophys Chem, Dept Neurobiol, Gottingen, Germany.;Max Delbruck Ctr Mol Med, Berlin Inst Med Syst Biol, Berlin, Germany.4 aut |
| 245 | 1 0 | a Clathrin coat controls synaptic vesicle acidification by blocking vacuolar ATPase activity |
| 264 | 1 | b ELIFE SCIENCES PUBLICATIONS LTD,c 2018 |
| 338 | a print2 rdacarrier | |
| 500 | a QC 20180514 | |
| 520 | a Newly-formed synaptic vesicles (SVs) are rapidly acidified by vacuolar adenosine triphosphatases (vATPases), generating a proton electrochemical gradient that drives neurotransmitter loading. Clathrin-mediated endocytosis is needed for the formation of new SVs, yet it is unclear when endocytosed vesicles acidify and refill at the synapse. Here, we isolated clathrin-coated vesicles (CCVs) from mouse brain to measure their acidification directly at the single vesicle level. We observed that the ATP-induced acidification of CCVs was strikingly reduced in comparison to SVs. Remarkably, when the coat was removed from CCVs, uncoated vesicles regained ATP-dependent acidification, demonstrating that CCVs contain the functional vATPase, yet its function is inhibited by the clathrin coat. Considering the known structures of the vATPase and clathrin coat, we propose a model in which the formation of the coat surrounds the vATPase and blocks its activity. Such inhibition is likely fundamental for the proper timing of SV refilling. | |
| 650 | 7 | a NATURVETENSKAPx Biologix Biofysik0 (SwePub)106032 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biophysics0 (SwePub)106032 hsv//eng |
| 700 | 1 | a Gowrisankaran, Sindhujau Univ Med Ctr Gottingen, European Neurosci Inst, Synapt Vesicle Dynam Grp, Gottingen, Germany.4 aut |
| 700 | 1 | a Krunic, Matijau Univ Med Ctr Gottingen, European Neurosci Inst, Synapt Vesicle Dynam Grp, Gottingen, Germany.4 aut |
| 700 | 1 | a Rammner, Burkhardu Sciloop, Hamburg, Germany.4 aut |
| 700 | 1 | a Woehler, Andrewu Max Delbruck Ctr Mol Med, Berlin Inst Med Syst Biol, Berlin, Germany.4 aut |
| 700 | 1 | a Lafer, Eileen M.u Univ Texas Hlth Sci Ctr San Antonio, Dept Biochem & Struct Biol, San Antonio, TX 78229 USA.;Univ Texas Hlth Sci Ctr San Antonio, Ctr Biomed Neurosci, San Antonio, TX 78229 USA.4 aut |
| 700 | 1 | a Mim, Carstenu KTH,Medicinteknik och hälsosystem4 aut0 (Swepub:kth)u1ce0qjf |
| 700 | 1 | a Jahn, Reinhardu Max Planck Inst Biophys Chem, Dept Neurobiol, Gottingen, Germany.4 aut |
| 700 | 1 | a Milosevic, Irau Univ Med Ctr Gottingen, European Neurosci Inst, Synapt Vesicle Dynam Grp, Gottingen, Germany.4 aut |
| 710 | 2 | a Max Planck Inst Biophys Chem, Dept Neurobiol, Gottingen, Germany.;Max Delbruck Ctr Mol Med, Berlin Inst Med Syst Biol, Berlin, Germany.b Univ Med Ctr Gottingen, European Neurosci Inst, Synapt Vesicle Dynam Grp, Gottingen, Germany.4 org |
| 773 | 0 | t eLIFEd : ELIFE SCIENCES PUBLICATIONS LTDg 7q 7x 2050-084X |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-227777 |
| 856 | 4 8 | u https://doi.org/10.7554/eLife.32569 |
| 856 | 4 8 | u http://kipublications.ki.se/Default.aspx?queryparsed=id:138152948 |
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