Search: (hsv:(TEKNIK OCH TEKNOLOGIER) hsv:(Industriell bioteknik)) lar1:(ki) >
Endonuclease specif...
Endonuclease specificity and sequence dependence of Type IIS restriction enzymes
-
- Lundin, Sverker (author)
- KTH,Genteknologi,Science for Life Laboratory, SciLifeLab
-
- Jemt, Anders (author)
- KTH,Genteknologi,Science for Life Laboratory, SciLifeLab
-
Finn, Terje-Hegge (author)
-
show more...
-
Foam, Napoleon (author)
-
Pettersson, Erik (author)
-
Käller, Max (author)
-
- Wirta, Valtteri (author)
- Karolinska Institutet
-
Lexow, Preben (author)
-
- Lundeberg, Joakim (author)
- KTH,Genteknologi,Science for Life Laboratory, SciLifeLab
-
show less...
-
(creator_code:org_t)
- 2015-01-28
- 2015
- English.
-
In: PLOS ONE. - : Public Library of Science. - 1932-6203. ; 10:1
- Related links:
-
https://doi.org/10.1...
-
show more...
-
https://journals.plo...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
http://kipublication...
-
show less...
Abstract
Subject headings
Close
- Restriction enzymes that recognize specific sequences but cleave unknown sequence outside the recognition site are extensively utilized tools in molecular biology. Despite this, systematic functional categorization of cleavage performance has largely been lacking. We established a simple and automatable model system to assay cleavage distance variation (termed slippage) and the sequence dependence thereof. We coupled this to massively parallel sequencing in order to provide sensitive and accurate measurement. With this system 14 enzymes were assayed (AcuI, BbvI, BpmI, BpuEI, BseRI, BsgI, Eco57I, Eco57MI, EcoP15I, FauI, FokI, GsuI, MmeI and SmuI). We report significant variation of slippage ranging from 1-54%, variations in sequence context dependence, as well as variation between isoschizomers. We believe this largely overlooked property of enzymes with shifted cleavage would benefit from further large scale classification and engineering efforts seeking to improve performance. The gained insights of in-vitro performance may also aid the in-vivo understanding of these enzymes.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Annan industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Other Industrial Biotechnology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
-
PLOS ONE
(Search for host publication in LIBRIS)
To the university's database