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LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00008021naa a2202089 4500
001oai:DiVA.org:sh-13911
003SwePub
008111214s2009 | |||||||||||000 ||eng|
024a https://urn.kb.se/resolve?urn=urn:nbn:se:sh:diva-139112 URI
024a https://doi.org/10.1016/j.ab.2008.11.0212 DOI
040 a (SwePub)sh
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
100a Rich, Rebecca L.4 aut
2451 0a A global benchmark study using affinity-based biosensors
264 1b Elsevier BV,c 2009
338 a print2 rdacarrier
520 a To explore the variability in biosensor studies, 150 participants from 20 countries were given the same protein samples and asked to determine kinetic rate constants for the interaction. We chose a protein system that was amenable to analysis using different biosensor platforms as well as by users of different expertise levels. The two proteins (a 50-kDa Fab and a 60-kDa glutathione S-transferase [GST] antigen) form a relatively high-affinity complex, so participants needed to optimize several experimental parameters, including ligand immobilization and regeneration conditions as well as analyte concentrations and injection/dissociation times. Although most participants collected binding responses that could be fit to yield kinetic parameters, the quality of a few data sets could have been improved by optimizing the assay design. Once these outliers were removed, the average reported affinity across the remaining panel of participants was 620 pM with a standard deviation of 980 pM. These results demonstrate that when this biosensor assay was designed and executed appropriately, the reported rate constants were consistent, and independent of which protein was immobilized and which biosensor was used.
650 7a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe
650 7a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng
700a Papalia, Giuseppe A.4 aut
700a Flynn, Peter J.4 aut
700a Furneisen, Jamie4 aut
700a Quinn, John4 aut
700a Klein, Joshua S.4 aut
700a Katsamba, Phini S.4 aut
700a Waddell, M. Brent4 aut
700a Scott, Michael4 aut
700a Thompson, Joshua4 aut
700a Berlier, Judie4 aut
700a Corry, Schuyler4 aut
700a Baltzinger, Mireille4 aut
700a Zeder-Lutzi, Gabrielle4 aut
700a Schoenemann, Andreas4 aut
700a Clabbers, Anca4 aut
700a Wieckowski, Sebastien4 aut
700a Murphy, Mary M.4 aut
700a Page, Phillip4 aut
700a Ryan, Thomas E.4 aut
700a Duffner, Jay4 aut
700a Ganguly, Tanmoy4 aut
700a Corbin, John4 aut
700a Gautam, Satyen4 aut
700a Anderluh, Gregor4 aut
700a Bavdek, Andrej4 aut
700a Reichmann, Dana4 aut
700a Yadav, Satya P.4 aut
700a Hommema, Eric4 aut
700a Pol, Ewa4 aut
700a Drake, Andrew4 aut
700a Klakamp, Scott4 aut
700a Chapman, Trevor4 aut
700a Kernaghan, Dawn4 aut
700a Miller, Ken4 aut
700a Schuman, Jason4 aut
700a Lindquist, Kevin4 aut
700a Herlihy, Kara4 aut
700a Murphy, Michael B.4 aut
700a Bohnsack, Richard4 aut
700a Andrien, Bruce4 aut
700a Brandani, Pietro4 aut
700a Terwey, Danny4 aut
700a Millican, Rohn4 aut
700a Darling, Ryan J.4 aut
700a Wang, Liann4 aut
700a Carter, Quincy4 aut
700a Dotzlaf, Joe4 aut
700a Lopez-Sagaseta, Jacinto4 aut
700a Campbell, Islay4 aut
700a Torreri, Paola4 aut
700a Hoos, Sylviane4 aut
700a England, Patrick4 aut
700a Liu, Yang4 aut
700a Abdiche, Yasmina4 aut
700a Malashock, Daniel4 aut
700a Pinkerton, Alanna4 aut
700a Wong, Melanie4 aut
700a Lafer, Eileen4 aut
700a Hinck, Cynthia4 aut
700a Thompson, Kevin4 aut
700a Di Primo, Carmelo4 aut
700a Joyce, Alison4 aut
700a Brooks, Jonathan4 aut
700a Torta, Federico4 aut
700a Hagel, Anne Birgitte Bagge4 aut
700a Krarup, Janus4 aut
700a Pass, Jesper4 aut
700a Ferreira, Monicau Södertörns högskola,Molekylärbiologi4 aut0 (Swepub:sh)SHMAFA
700a Shikov, Sergei4 aut
700a Mikolajczyk, Malgorzata4 aut
700a Abe, Yuki4 aut
700a Barbato, Gaetano4 aut
700a Giannetti, Anthony M.4 aut
700a Krishnamoorthy, Ganeshram4 aut
700a Beusink, Bianca4 aut
700a Satpaev, Daulet4 aut
700a Tsang, Tiffany4 aut
700a Fang, Eric4 aut
700a Partridge, James4 aut
700a Brohawn, Stephen4 aut
700a Horn, James4 aut
700a Pritsch, Otto4 aut
700a Obal, Gonzalo4 aut
700a Nilapwar, Sanjay4 aut
700a Busby, Ben4 aut
700a Gutierrez-Sanchez, Gerardo4 aut
700a Das Gupta, Ruchira4 aut
700a Canepa, Sylvie4 aut
700a Witte, Krista4 aut
700a Nikolovska-Coleska, Zaneta4 aut
700a Cho, Yun Hee4 aut
700a D'Agata, Roberta4 aut
700a Schlick, Kristian4 aut
700a Calvert, Rosy4 aut
700a Munoz, Eva M.4 aut
700a Hernaiz, Maria Jose4 aut
700a Bravman, Tsafir4 aut
700a Dines, Monica4 aut
700a Yang, Min-Hsiang4 aut
700a Puskas, Agnes4 aut
700a Boni, Erica4 aut
700a Li, Jiejin4 aut
700a Wear, Martin4 aut
700a Grinberg, Asya4 aut
700a Baardsnes, Jason4 aut
700a Dolezal, Olan4 aut
700a Gainey, Melicia4 aut
700a Anderson, Henrik4 aut
700a Peng, Jinlin4 aut
700a Lewis, Mark4 aut
700a Spies, Peter4 aut
700a Trinh, Quyhn4 aut
700a Bibikov, Sergei4 aut
700a Raymond, Jill4 aut
700a Yousef, Mohammed4 aut
700a Chandrasekaran, Vidya4 aut
700a Feng, Yuguo4 aut
700a Emerick, Anne4 aut
700a Mundodo, Suparna4 aut
700a Guimaraes, Rejane4 aut
700a McGirr, Katy4 aut
700a Li, Yue-Ji4 aut
700a Hughes, Heather4 aut
700a Mantz, Hubert4 aut
700a Skrabana, Rostislav4 aut
700a Witmer, Mark4 aut
700a Ballard, Joshua4 aut
700a Martin, Loic4 aut
700a Skladal, Petr4 aut
700a Korza, George4 aut
700a Laird-Offringa, Ite4 aut
700a Lee, Charlene S.4 aut
700a Khadir, Abdelkrim4 aut
700a Podlaski, Frank4 aut
700a Neuner, Phillippe4 aut
700a Rothacker, Julie4 aut
700a Rafique, Ashique4 aut
700a Dankbar, Nico4 aut
700a Kainz, Peter4 aut
700a Gedig, Erk4 aut
700a Vuyisich, Momchilo4 aut
700a Boozer, Christina4 aut
700a Ly, Nguyen4 aut
700a Toews, Mark4 aut
700a Uren, Aykut4 aut
700a Kalyuzhniy, Oleksandr4 aut
700a Lewis, Kenneth4 aut
700a Chomey, Eugene4 aut
700a Pak, Brian J.4 aut
700a Myszka, David G.4 aut
710a Södertörns högskolab Molekylärbiologi4 org
773t Analytical Biochemistryd : Elsevier BVg 386:2, s. 194-216q 386:2<194-216x 0003-2697x 1096-0309
856u https://europepmc.org/articles/pmc3793259?pdf=render
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:sh:diva-13911
8564 8u https://doi.org/10.1016/j.ab.2008.11.021

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Rich, Rebecca L.
Papalia, Giusepp ...
Flynn, Peter J.
Furneisen, Jamie
Quinn, John
Klein, Joshua S.
show more...
Katsamba, Phini ...
Waddell, M. Bren ...
Scott, Michael
Thompson, Joshua
Berlier, Judie
Corry, Schuyler
Baltzinger, Mire ...
Zeder-Lutzi, Gab ...
Schoenemann, And ...
Clabbers, Anca
Wieckowski, Seba ...
Murphy, Mary M.
Page, Phillip
Ryan, Thomas E.
Duffner, Jay
Ganguly, Tanmoy
Corbin, John
Gautam, Satyen
Anderluh, Gregor
Bavdek, Andrej
Reichmann, Dana
Yadav, Satya P.
Hommema, Eric
Pol, Ewa
Drake, Andrew
Klakamp, Scott
Chapman, Trevor
Kernaghan, Dawn
Miller, Ken
Schuman, Jason
Lindquist, Kevin
Herlihy, Kara
Murphy, Michael ...
Bohnsack, Richar ...
Andrien, Bruce
Brandani, Pietro
Terwey, Danny
Millican, Rohn
Darling, Ryan J.
Wang, Liann
Carter, Quincy
Dotzlaf, Joe
Lopez-Sagaseta, ...
Campbell, Islay
Torreri, Paola
Hoos, Sylviane
England, Patrick
Liu, Yang
Abdiche, Yasmina
Malashock, Danie ...
Pinkerton, Alann ...
Wong, Melanie
Lafer, Eileen
Hinck, Cynthia
Thompson, Kevin
Di Primo, Carmel ...
Joyce, Alison
Brooks, Jonathan
Torta, Federico
Hagel, Anne Birg ...
Krarup, Janus
Pass, Jesper
Ferreira, Monica
Shikov, Sergei
Mikolajczyk, Mal ...
Abe, Yuki
Barbato, Gaetano
Giannetti, Antho ...
Krishnamoorthy, ...
Beusink, Bianca
Satpaev, Daulet
Tsang, Tiffany
Fang, Eric
Partridge, James
Brohawn, Stephen
Horn, James
Pritsch, Otto
Obal, Gonzalo
Nilapwar, Sanjay
Busby, Ben
Gutierrez-Sanche ...
Das Gupta, Ruchi ...
Canepa, Sylvie
Witte, Krista
Nikolovska-Coles ...
Cho, Yun Hee
D'Agata, Roberta
Schlick, Kristia ...
Calvert, Rosy
Munoz, Eva M.
Hernaiz, Maria J ...
Bravman, Tsafir
Dines, Monica
Yang, Min-Hsiang
Puskas, Agnes
Boni, Erica
Li, Jiejin
Wear, Martin
Grinberg, Asya
Baardsnes, Jason
Dolezal, Olan
Gainey, Melicia
Anderson, Henrik
Peng, Jinlin
Lewis, Mark
Spies, Peter
Trinh, Quyhn
Bibikov, Sergei
Raymond, Jill
Yousef, Mohammed
Chandrasekaran, ...
Feng, Yuguo
Emerick, Anne
Mundodo, Suparna
Guimaraes, Rejan ...
McGirr, Katy
Li, Yue-Ji
Hughes, Heather
Mantz, Hubert
Skrabana, Rostis ...
Witmer, Mark
Ballard, Joshua
Martin, Loic
Skladal, Petr
Korza, George
Laird-Offringa, ...
Lee, Charlene S.
Khadir, Abdelkri ...
Podlaski, Frank
Neuner, Phillipp ...
Rothacker, Julie
Rafique, Ashique
Dankbar, Nico
Kainz, Peter
Gedig, Erk
Vuyisich, Momchi ...
Boozer, Christin ...
Ly, Nguyen
Toews, Mark
Uren, Aykut
Kalyuzhniy, Olek ...
Lewis, Kenneth
Chomey, Eugene
Pak, Brian J.
Myszka, David G.
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NATURAL SCIENCES
NATURAL SCIENCES
and Biological Scien ...
and Biochemistry and ...
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Analytical Bioch ...
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