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Sökning: WFRF:(Angelillo Scherrer Anne) > The Gas6-Axl Intera...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00004015naa a2200349 4500
001oai:lup.lub.lu.se:a33899c9-763a-41d5-b3b7-32b4b488e53b
003SwePub
008160411s2016 | |||||||||||000 ||eng|
0247 a https://lup.lub.lu.se/record/a33899c9-763a-41d5-b3b7-32b4b488e53b2 URI
0247 a https://doi.org/10.1074/jbc.M115.6990582 DOI
040 a (SwePub)lu
041 a engb eng
042 9 SwePub
072 7a art2 swepub-publicationtype
072 7a ref2 swepub-contenttype
1001 a Happonen, Kaisa Eu Lund University,Lunds universitet,Klinisk kemi, Malmö,Forskargrupper vid Lunds universitet,Clinical Chemistry, Malmö,Lund University Research Groups4 aut0 (Swepub:lu)med-khp
2451 0a The Gas6-Axl Interaction Mediates Endothelial Uptake of Platelet Microparticles
264 1c 2016
520 a Upon activation, platelets release plasma-membrane derived microparticles (PMPs) exposing phosphatidylserine (PS) on their surface. The function and clearance mechanism of these MPs are incompletely understood. As they are pro-coagulant and potentially pro-inflammatory, rapid clearance from the circulation is essential for prevention of thrombotic diseases. The tyrosine kinase receptors Tyro3, Axl and Mer (TAMs) and their ligands protein S and Gas6 are involved in the uptake of PS-exposing apoptotic cells in macrophages and dendritic cells. Both TAMs and their ligands are expressed in the vasculature, the functional significance of which is poorly understood. In this study we investigated how vascular TAMs and their ligands may mediate endothelial uptake of PMPs. PMPs, generated from purified human platelets, were isolated by ultracentrifugation and labeled with biotin or PKH67. The uptake of labeled MPs in the presence of protein S and Gas6 in human aortic endothelial cells (HAEC) and human umbilical vein endothelial cells (HUVEC) was monitored by flow cytometry, western blotting and confocal/electron microscopy. We found that both endothelial cell types can phagocytose PMPs, and using TAM-blocking antibodies or siRNA knock-down of individual TAMs we show that the uptake is mediated by endothelial Axl and Gas6. As circulating PMPs-levels were not altered in Gas6-/- mice compared to Gas6+/+ mice, we hypothesize that the Gas6-mediated uptake is not a means to clear the bulk of circulating PMPs but may serve to phagocytose PMPs locally generated at sites of platelet activation and as a way to affect endothelial responses.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Cell- och molekylärbiologi0 (SwePub)301082 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Cell and Molecular Biology0 (SwePub)301082 hsv//eng
7001 a Tran, Sinhu Lund University,Lunds universitet,Klinisk kemi, Malmö,Forskargrupper vid Lunds universitet,Clinical Chemistry, Malmö,Lund University Research Groups4 aut0 (Swepub:lu)klke-str
7001 a Mörgelin, Matthiasu Lund University,Lunds universitet,Infektionsmedicin,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Infection Medicine (BMC),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine4 aut0 (Swepub:lu)medk-mmn
7001 a Prince, Rajau Bern University Hospital4 aut
7001 a Calzavarini, Sarau Bern University Hospital4 aut
7001 a Angelillo-Scherrer, Anneu Bern University Hospital4 aut
7001 a Dahlbäck, Björnu Lund University,Lunds universitet,Klinisk kemi, Malmö,Forskargrupper vid Lunds universitet,Clinical Chemistry, Malmö,Lund University Research Groups4 aut0 (Swepub:lu)klke-bda
7102 a Klinisk kemi, Malmöb Forskargrupper vid Lunds universitet4 org
7730 t Journal of Biological Chemistryg 291:20, s. 10586-10601q 291:20<10586-10601x 1083-351X
8564 u http://dx.doi.org/10.1074/jbc.M115.699058y FULLTEXT
8564 8u https://lup.lub.lu.se/record/a33899c9-763a-41d5-b3b7-32b4b488e53b
8564 8u https://doi.org/10.1074/jbc.M115.699058

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