WFRF:(Asp Julia 1973 )
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 02445naa a2200505 4500 | |
| 001 | oai:gup.ub.gu.se/51030 | |
| 003 | SwePub | |
| 008 | 240528s2006 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://gup.ub.gu.se/publication/510302 URI |
| 040 | a (SwePub)gu | |
| 041 | a eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Asp, Julia,d 1973u Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för patologi,Institute of Biomedicine, Department of Pathology4 aut0 (Swepub:gu)xaspjd |
| 245 | 1 0 | a Nonradioactive in situ hybridization on frozen sections and whole mounts. |
| 264 | 1 | c 2006 |
| 520 | a Nonradioactive in situ hybridization offers a unique opportunity to study gene expression on samples with preserved histological information. This method makes it possible to locate not only where in a tissue a particular gene is expressed, but in many cases also in which specific cell type it is active. Here, we describe our current protocols for in situ hybridization on frozen sections or whole mounts of mouse embryos. The protocols included describe synthesis of a digoxigenin-labeled probe, tissue handling, hybridization of the probe to the mRNA expressed in the sample and signal detection. | |
| 653 | a Animals | |
| 653 | a Digoxigenin | |
| 653 | a metabolism | |
| 653 | a Embryo | |
| 653 | a cytology | |
| 653 | a Female | |
| 653 | a Frozen Sections | |
| 653 | a Gene Expression | |
| 653 | a Histological Techniques | |
| 653 | a In Situ Hybridization | |
| 653 | a methods | |
| 653 | a Mice | |
| 653 | a Molecular Probes | |
| 653 | a chemical synthesis | |
| 653 | a Nucleic Acid Hybridization | |
| 653 | a methods | |
| 653 | a Pregnancy | |
| 653 | a RNA Probes | |
| 653 | a chemical synthesis | |
| 653 | a RNA | |
| 653 | a Messenger | |
| 653 | a metabolism | |
| 700 | 1 | a Abramsson, Alexandra,d 1973u Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för patologi,Institute of Biomedicine, Department of Pathology4 aut0 (Swepub:gu)xabram |
| 700 | 1 | a Betsholtz, Christer,d 19594 aut |
| 710 | 2 | a Göteborgs universitetb Institutionen för biomedicin, avdelningen för patologi4 org |
| 773 | 0 | t Methods in molecular biology (Clifton, N.J.)g 326, s. 89-102q 326<89-102x 1064-3745 |
| 856 | 4 8 | u https://gup.ub.gu.se/publication/51030 |
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