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Sökning: WFRF:(Bouwmeester Harro J.) > Novel routes toward...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00003511naa a2200529 4500
001oai:DiVA.org:su-183551
003SwePub
008200727s2020 | |||||||||||000 ||eng|
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-1835512 URI
0247 a https://doi.org/10.1093/jxb/eraa0862 DOI
040 a (SwePub)su
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Jongedijk, Esmer4 aut
2451 0a Novel routes towards bioplastics from plants :b elucidation of the methylperillate biosynthesis pathway from Salvia dorisiana trichomes
264 c 2020-02-24
264 1b Oxford University Press (OUP),c 2020
338 a print2 rdacarrier
520 a Plants produce a large variety of highly functionalized terpenoids. Functional groups such as partially unsaturated rings and carboxyl groups provide handles to use these compounds as feedstock for biobased commodity chemicals. For instance, methylperillate, a monoterpenoid found in Salvia dorisiana, may be used for this purpose, as it carries both an unsaturated ring and a methylated carboxyl group. The biosynthetic pathway of methylperillate in plants is still unclear. In this work, we identified glandular trichomes from S. dorisiana as the location of biosynthesis and storage of methylperillate. mRNA from purified trichomes was used to identify four genes that can encode the pathway from geranyl diphosphate towards methylperillate. This pathway includes a (-)-limonene synthase (SdLS), a limonene 7-hydroxylase (SdL7H, CYP71A76), and a perillyl alcohol dehydrogenase (SdPOHDH). We also identified a terpene acid methyltransferase, perillic acid O-methyltransferase (SdPAOMT), with homology to salicylic acid OMTs. Transient expression in Nicotiana benthamiana of these four genes, in combination with a geranyl diphosphate synthase to boost precursor formation, resulted in production of methylperillate. This demonstrates the potential of these enzymes for metabolic engineering of a feedstock for biobased commodity chemicals.
650 7a NATURVETENSKAPx Biologi0 (SwePub)1062 hsv//swe
650 7a NATURAL SCIENCESx Biological Sciences0 (SwePub)1062 hsv//eng
653 a Biobased commodity chemicals
653 a glandular trichome
653 a limonene-7-hydroxylase
653 a methyl carboxyl ester
653 a methylperillate
653 a methyltransferase
653 a monoterpene biosynthesis
653 a Nicotiana benthamiana
653 a Salvia
7001 a Muller, Sebastian4 aut
7001 a van Dijk, Aalt D. J.4 aut
7001 a Schijlen, Elio4 aut
7001 a Champagne, Antoineu Stockholms universitet,Institutionen för ekologi, miljö och botanik,University of Louvain, Belgium4 aut
7001 a Boutry, Marc4 aut
7001 a Levisson, Mark4 aut
7001 a van der Krol, Sander4 aut
7001 a Bouwmeester, Harro4 aut
7001 a Beekwilder, Jules4 aut
7102 a Stockholms universitetb Institutionen för ekologi, miljö och botanik4 org
7730 t Journal of Experimental Botanyd : Oxford University Press (OUP)g 71:10, s. 3052-3065q 71:10<3052-3065x 0022-0957x 1460-2431
8564 u https://doi.org/10.1093/jxb/eraa086y Fulltext
8564 u https://academic.oup.com/jxb/article-pdf/71/10/3052/33325333/eraa086.pdf
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-183551
8564 8u https://doi.org/10.1093/jxb/eraa086

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