Sökning: WFRF:(Brisby Helena 1965) >
Indications of that...
Indications of that migration of stem cells is influenced by the extra cellular matrix architecture in the mammalian intervertebral disc region
-
- Barreto Henriksson, Helena (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för ortopedi,Institute of Clinical Sciences, Department of Orthopaedics
-
- Nicolaos, Papadimitriou, 1972 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för ortopedi,Institute of Clinical Sciences, Department of Orthopaedics
-
- Tschernitz, Sarah, 1994 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för ortopedi,Institute of Clinical Sciences, Department of Orthopaedics
-
visa fler...
-
- Svala, Emilia (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin,Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine,Inst för biomedicin och veterinär folkhälsovetenskap,Department of Biomedical Science and Veterinary Public Health,University of Gothenburg
-
- Skiöldebrand, Eva (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin,Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine,Inst för biomedicin och veterinär folkhälsovetenskap,Department of Biomedical Science and Veterinary Public Health,University of Gothenburg
-
- Windahl, Sara H, 1971 (författare)
- Gothenburg University,Göteborgs universitet,Centre for Bone and Arthritis Research
-
- Junevik, Katarina, 1965 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin,Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine
-
- Brisby, Helena, 1965 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för ortopedi,Institute of Clinical Sciences, Department of Orthopaedics
-
visa färre...
-
(creator_code:org_t)
-
- Elsevier BV, 2015
- 2015
- Engelska.
-
Ingår i: Tissue & Cell. - : Elsevier BV. - 0040-8166 .- 1532-3072. ; 47:5, s. 439-455
- Relaterad länk:
-
https://gup.ub.gu.se...
-
visa fler...
-
https://doi.org/10.1...
-
https://res.slu.se/i...
-
visa färre...
Abstract
Ämnesord
Stäng
- Disc-degeneration is believed a major cause for lumbar pain. Previously, potential stem cell niches in the intervertebral disc (IVD) region, located adjacent to epiphyseal plate was reported. The aim of the study was to examine migration of mesenchymal stem cells (MSCs), extracellular matrix (ECM) architecture in a potential cellular migration route (CMR; area located between the niche and IVD) and in the IVD in non-degenerated lapine- and in human degenerated IVD tissues. Human MSCs (n = 3), human degenerated IVD tissues (n = 10) and lapine IVDs (n = 10) were collected. The samples were examined by immunohistochemistry for stem cell markers; CD90, OCT3/4, pre-chondrocytic marker; GDF5, catabolic markers; MMP9, MMP13, inflammatory marker; IL1R, cellular migration markers; SNAI1, SNAI2, adhesion markers; β1-INTEGRIN and DDR2. In addition, gene-expression analyses (Real time PCR) were performed on additional samples. Further, time lapse studies were performed with hMSCs cultured on aligned COLL-I-fibers-coated glass-slides in DMEM-LG, 10% human serum containing fibroblast growth factor (bFGF). Results: Presence of stem cells (CD90+, OCT3/4 + ), pre-chondocytic cells (GDF5 + )and cells positive for migration markers (SNAI1+, SNAI2 + ), catabolic markers (MMP9+, MMP13 + ), inflammatory marker (IL1R + ), adhesion markers (DDR2+, B1-INTEGRIN + ) were detected (gene- and protein level) in investigated CMR and IVD regions. In the time lapse studies, MSCs alignment and protrusions were observed orientated in the same direction as collagen fibres. Results displays influence of ECM collagen architecture and collagen fiber spatial direction on migration of stem cells. The results can be useful when developing tissue-engineering strategies for disc-degeneration.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
Nyckelord
- mesenkymala stamceller
- cellmigration
- stamcellsnisch
- kollagen
- intervertebral disk
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas