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Purification, chara...
Purification, characterization, and biological compartmentalization of rat fetal antigen 1
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- Carlsson, Hans-Erik (författare)
- Uppsala universitet,Institutionen för fysiologi,Institutionen för försöksdjursvetenskap
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- Persdotter Hedlund, Gabriella (författare)
- Uppsala universitet,Institutionen för försöksdjursvetenskap,Institutionen för fysiologi
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- Fries, Erik (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Eriksson, Ulf J. (författare)
- Uppsala universitet,Institutionen för medicinsk cellbiologi
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- Hau, Jann (författare)
- Uppsala universitet,Institutionen för fysiologi,Institutionen för försöksdjursvetenskap
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(creator_code:org_t)
- Society for the Study of Reproduction, 2000
- 2000
- Engelska.
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Ingår i: Biology of Reproduction. - : Society for the Study of Reproduction. - 0006-3363 .- 1529-7268. ; 63:1, s. 30-33
- Relaterad länk:
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https://academic.oup...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- This study has established the rat as an animal model for the analysis of the biological role of fetal antigen 1 (FA1), a protein previously described in humans and mice. FA1 was purified from rat amniotic fluid by immunospecific affinity chromatography. Immunochemical identity between mouse and rat FA1 was established by crossed tandem immunoelectrophoresis. Molecular size was analyzed by mass spectrometry (33 kDa). The amino acid composition was determined, and the amino acid sequence was analyzed. The overall amino acid composition and sequence of the 28 first N-terminal amino acids were identical to the corresponding parts of rat preadipocyte factor 1 and rat adrenal zone glomerulosa protein. Extensive sequence similarity was found between rat and mouse FA1 (86%) and between rat and human FA1 (82%). The concentration of FA1 in fetal serum, maternal serum, urine, and amniotic fluid in rats was determined using an ELISA. The highest concentrations were found in fetal serum and amniotic fluid around Day 18 of pregnancy. This is the first report on the physicochemical characteristics and compartmentalization of rat FA1.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Nyckelord
- conceptus
- developmental biology
- pregnancy
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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