WFRF:(Dong Lan)
Search: WFRF:(Dong Lan) > (2010-2014) > Crystal structure o...
- 2 of 3
- Previous record
- Next record
- To hitlist
Crystal structure of tubulin folding cofactor A from Arabidopsis thaliana and its beta-tubulin binding characterization
- Lu, Lu (author)
-
- Nan, Jie (author)
- Lund University,Lunds universitet,MAX IV-laboratoriet,MAX IV Laboratory,Peking University
- Mi, Wei (author)
- show more...
- Li, Lan-Fen (author)
- Wei, Chun-Hong (author)
- Li, Yi (author)
- show less...
- (creator_code:org_t)
- 2010-07-16
- 2010
- English 7 s.
- In: FEBS Letters. - : Wiley. - 1873-3468 .- 0014-5793. ; 584:16, s. 9-3533
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Microtubules are composed of polymerized alpha/beta-tubulin heterodimers. Biogenesis of assembly-competent tubulin dimers is a complex multistep process that requires sequential actions of distinct molecular chaperones and cofactors. Tubulin folding cofactor A (TFCA), which captures beta-tubulin during the folding pathway, has been identified in many organisms. Here, we report the crystal structure of Arabidopsis thaliana TFC A (KIESEL, KIS), which forms a monomeric three-helix bundle. The functional binding analysis demonstrated that KIS interacts with beta-tubulin in plant. Furthermore, mutagenesis studies indicated that the alpha-helical regions of KIS participate in beta-tubulin binding. Unlike the budding yeast TFC A, the two loop regions of KIS are not required for this interaction suggesting a distinct binding mechanism of TFC A to beta-tubulin in plants.
Keyword
- Amino Acid Sequence
- Arabidopsis
- Arabidopsis Proteins
- Crystallography, X-Ray
- Genes, Plant
- Genetic Complementation Test
- Microtubule-Associated Proteins
- Models, Molecular
- Molecular Chaperones
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Plants, Genetically Modified
- Protein Binding
- Protein Folding
- Protein Interaction Domains and Motifs
- Protein Structure, Secondary
- Recombinant Proteins
- Sequence Homology, Amino Acid
- Tubulin
Publication and Content Type
- art (subject category)
- ref (subject category)
- 2 of 3
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Lu, Lu
- Nan, Jie
- Mi, Wei
- Li, Lan-Fen
- Wei, Chun-Hong
- Su, Xiao-Dong
- show more...
- Li, Yi
- show less...
- Articles in the publication
- FEBS Letters
- By the university
- Lund University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
