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  • Gilbert, M. Thomas P. (author)

Whole-genome shotgun sequencing of mitochondria from ancient hair shafts

  • Article/chapterEnglish2007

Publisher, publication year, extent ...

  • American Association for the Advancement of Science (AAAS),2007
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:uu-15309
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-15309URI
  • https://doi.org/10.1126/science.1146971DOI
  • https://urn.kb.se/resolve?urn=urn:nbn:se:nrm:diva-438URI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Although the application of sequencing-by-synthesis techniques to DNA extracted from bones has revolutionized the study of ancient DNA, it has been plagued by large fractions of contaminating environmental DNA. The genetic analyses of hair shafts could be a solution: We present 10 previously unexamined Siberian mammoth (Mammuthus primigenius) mitochondrial genomes, sequenced with up to 48-fold coverage. The observed levels of damage-derived sequencing errors were lower than those observed in previously published frozen bone samples, even though one of the specimens was >50,000 14C years old and another had been stored for 200 years at room temperature. The method therefore sets the stage for molecular-genetic analysis of museum collections.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Tomsho, Lynn P. (author)
  • Rendulic, Snjezana (author)
  • Packard, Michael (author)
  • Drautz, Daniela I. (author)
  • Sher, Andrei (author)
  • Tikhonov, Alexei (author)
  • Dalén, LoveNaturhistoriska riksmuseet,Enheten för bioinformatik och genetik(Swepub:nrm)lovedale (author)
  • Kuznetsova, Tatyana (author)
  • Kosintsev, Pavel (author)
  • Campos, Paula F. (author)
  • Higham, Thomas (author)
  • Collins, Matthew J. (author)
  • Wilson, Andrew S. (author)
  • Shidlovskiy, Fyodor (author)
  • Buigues, Bernard (author)
  • Ericson, Per G P,1956-Naturhistoriska riksmuseet,Forskningsavdelningen centralt(Swepub:nrm)pererics (author)
  • Germonpré, Mietje (author)
  • Götherström, AndersUppsala universitet,Evolutionsbiologi,Ancient DNA(Swepub:uu)angot030 (author)
  • Iacumin, Paola (author)
  • Nikolaev, Vladimir (author)
  • Nowak-Kemp, Malgosia (author)
  • Willerslev, Eske (author)
  • Knight, James R. (author)
  • Irzyk, Gerard P. (author)
  • Perbost, Clotilde S. (author)
  • Fredrikson, Karin M. (author)
  • Harkins, Timothy T. (author)
  • Sheridan, Sharon (author)
  • Miller, Webb (author)
  • Schuster, Stephan C. (author)
  • Naturhistoriska riksmuseetEnheten för bioinformatik och genetik (creator_code:org_t)

Related titles

  • In:Science: American Association for the Advancement of Science (AAAS)317:5846, s. 1927-19300036-80751095-9203

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