WFRF:(Holmqvist Peter)
Sökning: WFRF:(Holmqvist Peter) > A HupS-GFP fusion p...
A HupS-GFP fusion protein demonstrates a heterocyst-specific localization of the uptake hydrogenase in Nostoc punctiforme
-
- Camsund, Daniel (författare)
- Uppsala universitet,Molekylär biomimetik,Mikrobiell kemi
-
- Devine, Ellenor (författare)
- Uppsala universitet,Institutionen för fotokemi och molekylärvetenskap
-
- Holmqvist, Marie (författare)
- Uppsala universitet,Institutionen för fotokemi och molekylärvetenskap
- visa fler...
- visa färre...
- (creator_code:org_t)
- 2011-01-24
- 2011
- Engelska.
- Ingår i: FEMS Microbiology Letters. - : John Wiley & Sons. - 0378-1097 .- 1574-6968. ; 316:2, s. 152-159
- Tidskriftsartikel (refereegranskat)
Abstract
Ämnesord
Stäng
- All diazotrophic filamentous cyanobacteria contain an uptake hydrogenase that is involved in the reoxidation of H-2 produced during N-2-fixation. In Nostoc punctiforme ATCC 29133, N-2-fixation takes place in the microaerobic heterocysts, catalysed by a nitrogenase. Although the function of the uptake hydrogenase may be closely connected to that of nitrogenase, the localization in cyanobacteria has been under debate. Moreover, the subcellular localization is not understood. To investigate the cellular and subcellular localization of the uptake hydrogenase in N. punctiforme, a reporter construct consisting of the green fluorescent protein (GFP) translationally fused to HupS, within the complete hupSL operon, was constructed and transferred into N. punctiforme on a self-replicative vector by electroporation. Expression of the complete HupS-GFP fusion protein was confirmed by Western blotting using GFP antibodies. The N. punctiforme culture expressing HupS-GFP was examined using laser scanning confocal microscopy, and fluorescence was exclusively detected in the heterocysts. Furthermore, the fluorescence in mature heterocysts was localized to several small or fewer large clusters, which indicates a specificity of the subcellular localization of the uptake hydrogenase.
Nyckelord
- HupS
- GFP
- cyanobacteria
- fusion protein
- overlap-extension PCR
- Hup
- MEDICINE
- MEDICIN
- Medicin
- Medicine
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
- FEMS Microbiology Letters (Sök värdpublikationen i LIBRIS)
Till lärosätets databas
Hitta mer i SwePub
- Av författaren/redakt...
- Camsund, Daniel
- Devine, Ellenor
- Holmqvist, Marie
- Yohanoun, Peter
- Lindblad, Peter
- Stensjö, Karin
- Artiklar i publikationen
- FEMS Microbiolog ...
- Av lärosätet
- Uppsala universitet
Sök utanför SwePub
- Sök vidare i:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - Nationella bibliotekssystem
- LIBRIS.kb.se
