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Human mesenchymal s...
Human mesenchymal stem cells secrete hyaluronan-coated extracellular vesicles
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- Arasu, Uma Thanigai (författare)
- Institute of Biomedicine, University of Eastern Finland, Kuopio, Finland
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- Kärnä, Riikka (författare)
- Institute of Biomedicine, University of Eastern Finland, Kuopio, Finland
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- Härkönen, Kai (författare)
- Institute of Biomedicine, University of Eastern Finland, Kuopio, Finland
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- Oikari, Sanna (författare)
- Institute of Biomedicine, University of Eastern Finland, Kuopio, Finland
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- Koistinen, Arto (författare)
- SIB Labs, University of Eastern Finland, Kuopio, Finland
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- Kröger, Heikki (författare)
- Department of Orthopaedics and Traumatology, Kuopio University Hospital, Kuopio, Finland; Bone and Cartilage Research Unit, Surgery, Institute of Clinical Medicine, University of Eastern, Finland
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- Qu, Chengjuan, 1967- (författare)
- Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB),Chondrogenic and Osteogenic Differentiation Group
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- Lammi, Mikko, 1961- (författare)
- Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB),School of Public Health, Health Science Center of Xi'an Jiaotong University, Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, Xi'an, PR China,Chondrogenic and Osteogenic Differentiation Group
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- Rilla, Kirsi (författare)
- Institute of Biomedicine, University of Eastern Finland, Kuopio, Finland
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(creator_code:org_t)
- Amsterdam : Elsevier, 2017
- 2017
- Engelska.
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Ingår i: Matrix Biology. - Amsterdam : Elsevier. - 0945-053X .- 1569-1802. ; 64, s. 54-68
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Extracellular vesicles (EVs) secreted by stem cells are potential factors mediating tissue regeneration. They travel from bone marrow stem cells into damaged tissues, suggesting that they can repair tissue injuries without directly replacing parenchymal cells. We have discovered that hyaluronan (HA) synthesis is associated with the shedding of HA-coated EVs. The aim of this study was to test whether bone marrow-derived hMSCs secrete HA-coated EVs. The EVs secreted by MSCs were isolated by differential centrifugation and characterized by nanoparticle tracking analysis. Their morphology and budding mechanisms were inspected by confocal microscopy and correlative light and electron microscopy. Hyaluronan synthesis of hMSCs was induced by lipopolysaccharide and inhibited by RNA interference and 4-methylumbelliferone. It was found that the MSCs have extremely long apical and lateral HA-coated filopodia, typical for cells with an active HA secretion. Additionally, they secreted HA-coated EVs carrying mRNAs for CD44 and all HAS isoforms. The results show that stem cells have a strong intrinsic potential for HA synthesis and EV secretion, and the amount of HA carried on EVs reflects the HA content of the original cells. These results show that the secretion of HA-coated EVs by hMSCs is a general process, that may contribute to many of the mechanisms of HA-mediated tissue regeneration. Additionally, an HA coat on EVs may regulate their interactions with target cells and participate in extracellular matrix remodeling.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- CD44
- extracellular vesicle
- filopodia
- hyaluronan
- hyaluronan synthase
- stem cell
- cellforskning
- cell research
- molekylär cellbiologi
- molecular cell biology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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- Av författaren/redakt...
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Arasu, Uma Thani ...
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Kärnä, Riikka
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Härkönen, Kai
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Oikari, Sanna
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Koistinen, Arto
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Kröger, Heikki
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Qu, Chengjuan, 1 ...
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Lammi, Mikko, 19 ...
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Rilla, Kirsi
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- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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- Artiklar i publikationen
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Matrix Biology
- Av lärosätet
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Umeå universitet