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Sökning: WFRF:(Kurkus Jan) > (1993-1994) > Genotyping of hepat...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00003736naa a2200445 4500
001oai:lup.lub.lu.se:eeecb376-4fe2-44c5-8ab0-0fd27de022cf
003SwePub
008160401s1994 | |||||||||||000 ||eng|
0247 a https://lup.lub.lu.se/record/11084882 URI
0247 a https://doi.org/10.1002/jmv.18904403112 DOI
040 a (SwePub)lu
041 a engb eng
042 9 SwePub
072 7a art2 swepub-publicationtype
072 7a ref2 swepub-contenttype
1001 a Widell, Andersu Lund University,Lunds universitet,Klinisk mikrobiologi, Malmö,Forskargrupper vid Lunds universitet,Clinical Microbiology, Malmö,Lund University Research Groups4 aut0 (Swepub:lu)mikr-awi
2451 0a Genotyping of hepatitis C virus isolates by a modified polymerase chain reaction assay using type specific primers: epidemiological applications
264 1b Wiley,c 1994
520 a A polymerase chain reaction (PCR)-based assay using primers against the hepatitis C core gene has been described [Okamoto et al. (1992a): Journal of General Virology 73:673-679]. Within the two major HCV genotypes 1 and 2, the Okamoto system identifies two subtypes each (1a, 1b and 2a, 2b, respectively). Typing is achieved by a primary PCR with consensus primers followed by a nested PCR with type specific primers. The original assay was modified by addition of a parallel second PCR identifying the recently described major genotype 3. The assay also identifies in duplicate subtype 1b (type II by Okamoto), suggested to respond poorly to interferon. Reaction conditions were reviewed and melting temperatures of all typing primers equalised to increase strigency. The modified system functioned well and typing results were supported by partial core sequencing. The following distribution of genotypes was found in 53 hepatitis C virus (HCV) infected Swedish blood donors: genotype 1a (57%), 3 (19%), 1b (13%), and 2b (11%). In six recipients of HCV infected blood identified in a retrospective study, the recipient HCV genotype was identical to donor HCV genotype. Furthermore, in HCV positive couples identical genotype was observed when only one partner had an external risk factor; whereas genotypes were often diverse if both sex partners had parenteral risk factors. Finally, a cluster of hepatitis C cases in a haemodialysis unit was evaluated retrospectively. Eight patients had genotype 1b, two had mixed 1a and 1b, and one had type 1a. The modified HCV genotyping assay was of value in examining different epidemiological situations and can be expanded presumably to include future genotypes.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Mikrobiologi inom det medicinska området0 (SwePub)301092 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Microbiology in the medical area0 (SwePub)301092 hsv//eng
653 a molecular epidemiology
653 a sexual transmission
653 a nosocomial infection
653 a HCV-infected blood donor
653 a haemodialysis infection
7001 a Shev, Steven4 aut
7001 a Månsson, Siv4 aut
7001 a Zhang, Yong-Yuan4 aut
7001 a Foberg, Ulla4 aut
7001 a Norkrans, Gunnar4 aut
7001 a Fryden, Aril4 aut
7001 a Weiland, Ola4 aut
7001 a Kurkus, Jan4 aut
7001 a Nordenfelt, Erik4 aut
7102 a Klinisk mikrobiologi, Malmöb Forskargrupper vid Lunds universitet4 org
7730 t Journal of Medical Virologyd : Wileyg 44:3, s. 272-279q 44:3<272-279x 1096-9071x 0146-6615
8564 u http://dx.doi.org/10.1002/jmv.1890440311y FULLTEXT
8564 8u https://lup.lub.lu.se/record/1108488
8564 8u https://doi.org/10.1002/jmv.1890440311

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