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Immunoassays distin...
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Martensson, JohanKarolinska Institutet
(author)
Immunoassays distinguishing between HNL/NGAL released in urine from kidney epithelial cells and neutrophils
- Article/chapterEnglish2012
Publisher, publication year, extent ...
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Elsevier BV,2012
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:uu-181994
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-181994URI
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https://doi.org/10.1016/j.cca.2012.05.010DOI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:125122254URI
Supplementary language notes
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Language:English
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Summary in:English
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Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Background: The distinction between monomeric human neutrophil lipocalin/neutrophil gelatinase-associated lipocalin (HNL/NGAL), secreted by injured kidney tubular cells, and dimeric HNL/NGAL, released by activated neutrophils, is important to accurately diagnose acute kidney injury (AKI).Methods: 132 urine samples from 44 intensive care unit (ICU) patients and five urine samples from non-ICU patients with urinary tract infections (UTIs) were analyzed by two monoclonal enzyme-linked immunosorbent assays (ELISA-1 and ELISA-2). The presence of monomeric and/or dimeric HNL/NGAL in each sample was visualized by Western blotting.Results: The ELISA-1 detected both monomeric and dimeric HNL/NGAL whereas the ELISA-2 almost exclusively detected dimeric HNL/NGAL with an area under the receiver-operating characteristics curve (AuROC) of 0.90. The ELISA-1/ELISA-2 ratio detected the monomeric form with an AuROC of 0.92. In 32 AKI patients, dimer-specific EUSA-2 levels decreased pre-AKI whereas the monomer-specific ELISA-1/ELISA-2 ratio gradually increased beyond AKI diagnosis. High EUSA-2 levels and/or low ELISA-1/ELISA-2 ratios detected a predominance of dimeric HNL/NGAL in urine from the patients with tills.Conclusions: In combination, our two ELISAs distinguish monomeric HNL/NGAL, produced by the kidney epithelium, from dimeric HNL/NGAL, released by neutrophils during AKI development, as well as reduce the confounding effect of neutrophil involvement when bacteriuria is present.
Subject headings and genre
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Acute kidney injury
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AKIN
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Biomarker
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HNL
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NGAL
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RIFLE
Added entries (persons, corporate bodies, meetings, titles ...)
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Xu, ShengyuanUppsala universitet,Biokemisk struktur och funktion(Swepub:uu)shengyuanxu
(author)
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Bell, MaxKarolinska Institutet
(author)
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Martling, Claes-RolandKarolinska Institutet
(author)
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Venge, PerUppsala universitet,Biokemisk struktur och funktion(Swepub:uu)pervenge
(author)
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Karolinska InstitutetBiokemisk struktur och funktion
(creator_code:org_t)
Related titles
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In:Clinica Chimica Acta: Elsevier BV413:19-20, s. 1661-16670009-89811873-3492
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