WFRF:(Miraglia E)
Sökning: WFRF:(Miraglia E) > (2014) > Boosting innate imm...
LIBRIS Formathandbok (Information om MARC21)
| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 02739naa a2200325 4500 | |
| 001 | oai:prod.swepub.kib.ki.se:128689402 | |
| 003 | SwePub | |
| 008 | 240701s2014 | |||||||||||000 ||eng| | |
| 024 | 7 | a http://kipublications.ki.se/Default.aspx?queryparsed=id:1286894022 URI |
| 024 | 7 | a https://doi.org/10.1177/17534259134933382 DOI |
| 040 | a (SwePub)ki | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Nylen, Fu Karolinska Institutet4 aut |
| 245 | 1 0 | a Boosting innate immunity: development and validation of a cell-based screening assay to identify LL-37 inducers |
| 264 | c 2013-07-24 | |
| 264 | 1 | b SAGE Publications,c 2014 |
| 520 | a Innate immunity, the front line of our defence against pathogens, relies, to a great extent, on the production of antimicrobial peptides (AMPs). These peptides exhibit antimicrobial activity and immunomodulatory properties. In humans, AMPs include the defensins (α- and β-families) and the cathelicidin, LL-37. Bacterial resistance against antibiotics is a growing concern, and novel antimicrobial strategies are needed urgently. Hence, the concept of strengthening immune defences against infectious microbes by inducing AMP expression may represent novel or complementary pharmaceutical interventions in the treatment or prevention of infections. We have developed and validated a robust cell-based reporter assay for LL-37 expression, which serves as a marker for a healthy epithelial barrier. This reporter assay can be a powerful tool for high-throughput screenings. We first employed our assay to screen a panel of histone deacetylase inhibitors and derivatives, and then the Prestwick Chemical Library of Food and Drug Administration-approved compounds. After hit confirmation and independent validation in the parental cell line we identified five novel inducers of LL-37. This reporter assay will help to identify novel drug candidates for the treatment and prevention of infections. Importantly, the pattern of hits obtained may suggest cellular pathways and key mediators involved in the regulation of AMP expression. | |
| 700 | 1 | a Miraglia, Eu Karolinska Institutet4 aut |
| 700 | 1 | a Cederlund, A4 aut |
| 700 | 1 | a Ottosson, Hu Karolinska Institutet4 aut |
| 700 | 1 | a Stromberg, Ru Karolinska Institutet4 aut |
| 700 | 1 | a Gudmundsson, GH4 aut |
| 700 | 1 | a Agerberth, Bu Karolinska Institutet4 aut |
| 710 | 2 | a Karolinska Institutet4 org |
| 773 | 0 | t Innate immunityd : SAGE Publicationsg 20:4, s. 364-376q 20:4<364-376x 1753-4267x 1753-4259 |
| 856 | 4 8 | u http://kipublications.ki.se/Default.aspx?queryparsed=id:128689402 |
| 856 | 4 8 | u https://doi.org/10.1177/1753425913493338 |
Hitta mer i SwePub
- Av författaren/redakt...
- Nylen, F
- Miraglia, E
- Cederlund, A
- Ottosson, H
- Stromberg, R
- Gudmundsson, GH
- visa fler...
- Agerberth, B
- visa färre...
- Artiklar i publikationen
- Innate immunity
- Av lärosätet
- Karolinska Institutet
Sök utanför SwePub
- Sök vidare i:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - Nationella bibliotekssystem
- LIBRIS.kb.se
