Sökning: WFRF:(Mironov Andrey) >
Heparan sulfates re...
Heparan sulfates regulate axonal excitability and context generalization through CA2+/Calmodulin-dependent protein kinase II
-
- Song, Inseon (författare)
- Molecular Neuroplasticity Group, German Center for Neurodegenerative Diseases (DZNE), Magdeburg, Germany
-
- Kuznetsova, Tatiana (författare)
- Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB)
-
- Baidoe-Ansah, David (författare)
- Molecular Neuroplasticity Group, German Center for Neurodegenerative Diseases (DZNE), Magdeburg, Germany
-
visa fler...
-
- Mirzapourdelavar, Hadi (författare)
- Molecular Neuroplasticity Group, German Center for Neurodegenerative Diseases (DZNE), Magdeburg, Germany
-
- Senkov, Oleg (författare)
- Molecular Neuroplasticity Group, German Center for Neurodegenerative Diseases (DZNE), Magdeburg, Germany
-
- Hayani, Hussam (författare)
- Molecular Neuroplasticity Group, German Center for Neurodegenerative Diseases (DZNE), Magdeburg, Germany
-
- Mironov, Andrey (författare)
- Molecular Neuroplasticity Group, German Center for Neurodegenerative Diseases (DZNE), Magdeburg, Germany
-
- Kaushik, Rahul (författare)
- Molecular Neuroplasticity Group, German Center for Neurodegenerative Diseases (DZNE), Magdeburg, Germany
-
- Druzin, Michael, 1971- (författare)
- Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB)
-
- Johansson, Staffan, 1962- (författare)
- Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB)
-
- Dityatev, Alexander (författare)
- Molecular Neuroplasticity Group, German Center for Neurodegenerative Diseases (DZNE), Magdeburg, Germany; Medizinische Fakultät, Otto-von-Güricke-Universität Magdeburg, Magdeburg, Germany; Center for Behavioral Brain Sciences (CBBS), Magdeburg, Germany
-
visa färre...
-
(creator_code:org_t)
- 2023-02-25
- 2023
- Engelska.
-
Ingår i: Cells. - : MDPI. - 2073-4409. ; 12:5
- Relaterad länk:
-
https://doi.org/10.3...
-
visa fler...
-
https://umu.diva-por... (primary) (Raw object)
-
https://urn.kb.se/re...
-
https://doi.org/10.3...
-
visa färre...
Abstract
Ämnesord
Stäng
- Our previous studies demonstrated that enzymatic removal of highly sulfated heparan sulfates with heparinase 1 impaired axonal excitability and reduced expression of ankyrin G at the axon initial segments in the CA1 region of the hippocampus ex vivo, impaired context discrimination in vivo, and increased Ca2+/calmodulin-dependent protein kinase II (CaMKII) activity in vitro. Here, we show that in vivo delivery of heparinase 1 in the CA1 region of the hippocampus elevated autophosphorylation of CaMKII 24 h after injection in mice. Patch clamp recording in CA1 neurons revealed no significant heparinase effects on the amplitude or frequency of miniature excitatory and inhibitory postsynaptic currents, while the threshold for action potential generation was increased and fewer spikes were generated in response to current injection. Delivery of heparinase on the next day after contextual fear conditioning induced context overgeneralization 24 h after injection. Co-administration of heparinase with the CaMKII inhibitor (autocamtide-2-related inhibitory peptide) rescued neuronal excitability and expression of ankyrin G at the axon initial segment. It also restored context discrimination, suggesting the key role of CaMKII in neuronal signaling downstream of heparan sulfate proteoglycans and highlighting a link between impaired CA1 pyramidal cell excitability and context generalization during recall of contextual memories.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Nyckelord
- ankyrin G
- axon initial segment
- axonal excitability
- context discrimination
- extracellular matrix
- synaptic plasticity
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
-
Cells
(Sök värdpublikationen i LIBRIS)
Till lärosätets databas
- Av författaren/redakt...
-
Song, Inseon
-
Kuznetsova, Tati ...
-
Baidoe-Ansah, Da ...
-
Mirzapourdelavar ...
-
Senkov, Oleg
-
Hayani, Hussam
-
visa fler...
-
Mironov, Andrey
-
Kaushik, Rahul
-
Druzin, Michael, ...
-
Johansson, Staff ...
-
Dityatev, Alexan ...
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Medicinska och f ...
-
och Neurovetenskaper
- Artiklar i publikationen
-
Cells
- Av lärosätet
-
Umeå universitet