Sökning: WFRF:(Ohlsson Claes J.)
> (1995-1999) >
Growth hormone rece...
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Slootweg, M C
(författare)
Growth hormone receptor activity is stimulated by insulin-like growth factor binding protein 5 in rat osteosarcoma cells.
- Artikel/kapitelEngelska1996
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Nummerbeteckningar
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LIBRIS-ID:oai:gup.ub.gu.se/91469
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https://gup.ub.gu.se/publication/91469URI
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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Osteoblast-like UMR-106.01 rat osteosarcoma cells express high affinity growth hormone (GH) receptors (GHRs). Because osteoblasts secrete insulin-like growth factor binding protein-5 (IGFBP-5), we evaluated whether it also modulates GH binding and GHR expression in UMR cells. Human recombinant intact IGFBP-5 stimulated 125I-hGH binding in a dose-dependent manner (dose range 300-3000 ng/ml), inducing an increase to 193.6 +/- 2.1% of control binding at 3000 ng/ml (P < 0.001). Carboxy-truncated IGFBP-5 also stimulated GH binding but with less potency (125 +/- 2.7% of control at 3000 ng/ml, P < 0.01). GHRs identified by chemical crosslinking of 125I-hGH to cell monolayers increased after treatment with IGFBP-5 and decreased in response to insulin-like growth factor-I (IGF-I). GHR mRNA levels, as quantitated by a solution hybridization RNAse protection assay, increased up to 3 to 7-fold in a time-dependent manner by intact IGFBP-5 but not by carboxy-truncated IGFBP-5. An antiserum to IGFBP-5 reduced basal GH binding to 56.7 +/- 4.3% of control value at a concentration of 0.5% (P < 0.001), showing that IGFBP-5 produced by the cells is a strong regulator of GH binding. IGFBP-5 antiserum also decreased GH binding to 85.9 +/- 0.9% of IGFBP-5 stimulated value (P < 0.001), showing the specificity of IGFBP-5 stimulation. To determine whether the GHR upregulation was physiologically significant, cell proliferation was evaluated after coincubation of IGFBP-5 with low, non-stimulatory concentrations of GH. IGFBP-5 (1000 ng/ml) induced cell proliferation to 116.2 +/- 3.2% of control levels, and coincubation with hGH at 10 ng/ml induced an increase to 133.3 +/- 0.1% of control levels. We conclude that exogenous and endogenous IGFBP-5 upregulate GHR mRNA levels and GH binding and this interaction potentiates GH-stimulated mitogenesis in osteoblastic cells.
Ämnesord och genrebeteckningar
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Animals
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Bone Neoplasms
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metabolism
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Cell Division
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drug effects
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Cell Line
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Cross-Linking Reagents
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Growth Hormone
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pharmacology
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Humans
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Insulin-Like Growth Factor Binding Protein 5
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pharmacology
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Iodine Radioisotopes
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diagnostic use
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Isotope Labeling
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Osteosarcoma
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metabolism
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Radioligand Assay
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Rats
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Receptors
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Somatotropin
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drug effects
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Ribonucleases
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metabolism
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Tumor Cells
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Cultured
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Ohlsson, Claes,1965Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för internmedicin,Institute of Internal Medicine, Dept of Medicine(Swepub:gu)xohlcl
(författare)
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van Elk, E J
(författare)
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Netelenbos, J C
(författare)
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Andress, D L
(författare)
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Göteborgs universitetInstitutionen för invärtesmedicin, Avdelningen för internmedicin
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Growth regulation6:4, s. 238-460956-523X
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