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Analysis of IG/TCR ...
Analysis of IG/TCR gene rearrangements in Swedish childhood acute lymphoblastic leukemia diagnosed 2002-2006: a multi-centre study supporting the applicability of real-time-PCR for minimal residual disease assessment
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- Thörn, Ingrid, 1957- (författare)
- Uppsala universitet,Institutionen för genetik och patologi,Hematologi och immunologi,Molecular Hematology
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- Forestier, Erik (författare)
- Department of Clinical Sciences, Paediatrics, Umeå University, Umeå, Sweden
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- Thuresson, Britt (författare)
- Division of Hematology and Transfusion Medicine, Department of Laboratory Medicine, Lund University, Lund, Sweden
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- Wasslavik, Carina (författare)
- Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska University Hospital, Gothenburg, Sweden
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- Malec, Maria (författare)
- Department of Pathology, Karolinska University Hospital, Stockholm, Sweden
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- Li, Aihong (författare)
- Department of Biosciences, Clinical Chemistry, Umeå University, Umeå,
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- Lindström-Eriksson, Elenor (författare)
- Department of Medical Biosciences, Pathology, Umeå University, Umeå, Sweden
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- Botling, Johan (författare)
- Uppsala universitet,Institutionen för genetik och patologi,Hematologi och immunologi,Molecular Hematology
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- Barbany, Gisela (författare)
- Uppsala universitet,Institutionen för genetik och patologi
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- Jacobsson, Stefan (författare)
- Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska University Hospital, Gothenburg, Sweden
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- Olofsson, Tor (författare)
- Division of Hematology & Transfusion Medicine, Department of Laboratory Medicine, Lund University, Lund, Sweden
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- Porwit, Anna (författare)
- Department of Pathology, Karolinska University Hospital, Stockholm, Sweden
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- Sundström, Christer (författare)
- Uppsala universitet,Institutionen för genetik och patologi,Hematologi och immunologi,Molecular Hematology
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- Rosenquist, Richard (författare)
- Uppsala universitet,Institutionen för genetik och patologi,Hematologi och immunologi,Molecular Hematology
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(creator_code:org_t)
- Engelska.
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- Minimal residual disease (MRD) detection during the early treatment phase has become an important stratification parameter in many childhood acute lymphoblastic leukemia (ALL) treatment protocols. Here we aimed to address the applicability of real-time quantitative polymerase chain reaction (RQ-PCR) of rearranged antigen receptor genes as an MRD method in a multi-centre setting. From a Swedish population-based cohort of 334 ALL cases diagnosed during 2002-2006, we analysed 279 diagnostic samples (84%) by screening for rearranged immunoglobulin (IG) and T-cell receptor (TCR) genes. Allele-specific oligonucleotides were designed for each patient rearrangement, and the sensitivity and quantitative level was determined for each target. The analyses were performed at five different centres while interpretation of the results was performed at consensus meetings. Overall, clonal IG/TCR rearrangements were detected in 97% (236/244) of B-cell precursor ALL (BCP ALL) and 94% (33/35) of T-ALL. A sensitive RQ-PCR analysis (≤ 10-4) was obtained in 89% (216/244) of BCP ALL and in 74% (26/35) of T-ALL. With the stratification threshold of ≥10-3 for identification of high-risk patients, 93% of BCP ALL and 86% of T-ALL reached this quantitative range by at least one target gene. Taken together, this national multi-centre study supports the use of RQ-PCR analysis as a robust method for MRD detection in the majority of childhood ALL cases.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Hematologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Hematology (hsv//eng)
Nyckelord
- RQ-PCR
- rearranged IG/TCR genes
- minimal residual disease
- childhood acute lymphoblastic leukemia
- Haematology
- Hematologi
Publikations- och innehållstyp
- vet (ämneskategori)
- ovr (ämneskategori)
- Av författaren/redakt...
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Thörn, Ingrid, 1 ...
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Forestier, Erik
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Thuresson, Britt
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Wasslavik, Carin ...
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Malec, Maria
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Li, Aihong
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visa fler...
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Lindström-Erikss ...
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Botling, Johan
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Barbany, Gisela
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Jacobsson, Stefa ...
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Olofsson, Tor
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Porwit, Anna
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Sundström, Chris ...
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Rosenquist, Rich ...
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Hematologi
- Av lärosätet
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Uppsala universitet